核型多角体病毒对棉铃虫酯酶和酚氧化酶活性的影响  

The Effect of NPV on Esterase and Phenoloxidase Activities from Helicoverpa armigera (Hubner) Hemolymph

作　　者：王晓容[1] 邓海滨[1,2] 匡石滋[1] 吴洁芳[1] 邢巧梅 连书亭 

机构地区：[1]广东省农业科学院果树研究所,广州510640 [2]中国科学院华南植物研究所,广州510650 [3]河南省禹州市生物农药厂,禹州456570

出　　处：《华中农业大学学报》2003年第6期553-556,共4页Journal of Huazhong Agricultural University

基　　金：河南省高新技术产业化重点项目[豫高新(2001)5号];广东省星火计划项目(2003B21602)资助

摘　　要：对感染核型多角体病毒(nuclear polyhedrosis,NPV)的棉铃虫Helicoverpa armigera(Hubner)幼虫血淋巴进行了酯酶和酚氧化酶电泳分析。结果表明:2种酶带在感染12 h后其活力发生了明显变化;酯酶酶带2 d后发生位移,酚氧化酶酶带5 d发生位移,且各自建立新的酶系统。酚氧化酶比活力分析结果表明:感染NPV后,1 d达到最高点,2～3 d处于最低点,4 d后回升。This paper reported the electrophoresie assay of esterase and phenoloxidase activity in Helicoverpa armigera (Hubner) hemolymph which was infected with nuclear polyhedrosis virus (NPV). The result showed as follows that after being infected for 12 h, the activity of these two emzyme bands changed obviously, and the position of esterase bands shifted at 2 d, the phenoloxidase bands shifted at 5 d, and new enzyme systems presented. The result of phenoloxidase activity assay showed that infected by NPV, the activity reached the maximum at 1 d, reached the minimum between 2 d and 3 d,and rise again at 4 d.

关 键 词：核型多角体病毒 棉铃虫 酯酶 酚氧化酶 酶活性 影响因素 

分 类 号：S435.622[农业科学—农业昆虫与害虫防治]