超临界流体萃取高效液相色谱法测定总合草苔虫中草苔虫内酯4含量  

Determination of bryostatin 4 in Bugula neritina by HPLC and supercritical fluid extraction

作　　者：孙鹏[1] 李玲[1] 易杨华[1] 张淑瑜[1] 汤海峰[1] 周大铮[1] 

机构地区：[1]第二军医大学药学院海洋药物研究室,上海200433

出　　处：《中国药学杂志》2003年第12期907-909,共3页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金 ( 2 9672 0 5 0 );上海市科委新药基金重点项目 ( 0 14 3 193 2 0 )

摘　　要：目的　建立超临界流体萃取 (SFE)和高效液相色谱 (HPLC)的联用技术测定总合草苔虫中抗癌活性成分的方法。方法　正交设计法考察了影响草苔虫内酯 4 (bryostatin 4 )超临界流体萃取效率的主次效应及最佳萃取条件 ,并以HPLC定量检测bryostatin 4。结果　SFE萃取的最佳条件为 :压力 4 0MPa ,温度 4 5℃ ,改性剂量 1mL ,萃取体积 15mL。结论　SFE HPLC可用于检测总合草苔虫中的草苔虫内酯成分 ,该方法快速。OBJECTIVE: To determine the bryostatin 4 in Bugula neritina on the basis of SFE and HPLC. METHODS: Orthogonal design and analysis of variance were used to optimize the four variables (temperature, pressure, volume of modifier and extraction volume) respectively. The bryostatin 4 in SFE extracts was determined by HPLC. RESULTS: The optimized conditions were as follows: pressure 40 MPa, temperature 45°C, 1 mL chloroform as modifier and extraction volume of 15 mL. The recovery of bryostatin 4 was 95.4% with RSD 0.64%. CONCLUSION: Our work provided a new way to detect the antinoeplastic components in Bugula neritina. SFE is rapid, convenient and less solvent consuming compared with solvent extraction.

关 键 词：超临界流体萃取 高效液相色谱法 测定 总合草苔虫 草苔虫内酯 含量 

分 类 号：R284[医药卫生—中药学]