喷昔洛韦对单纯疱疹病毒感染小鼠的治疗作用  

Effect of penciclovir on experimental mouse model infected by herpes simplex virus

作　　者：侯炜[1] 杨占秋[1] 曲虹[2] 肖红[1] 文利[1] 熊增慧[3] 刘红[3] 

机构地区：[1]武汉大学医学院病毒研究所,湖北武汉430071 [2]华中科技大学同济医学院微生物教研室,湖北武汉430060 [3]湖北省医药工业研究院,湖北武汉430000

出　　处：《中国药学杂志》2003年第12期921-924,共4页Chinese Pharmaceutical Journal

摘　　要：目的　研究喷昔洛韦 (PCV)对单纯疱疹病毒感染小鼠的疗效。方法　用病毒滴度为 10 0LD50 /0 .1mLHSV 1,HSV 2每只分别腹腔注射 0 .0 3mL ,2 4h后皮下注射给药 ,PCV剂量分别为 2 0 ,5 0 ,10 0 ,2 5 0mg·kg-1·d-1,疗程 5d。观察动物的反应 ,出现死亡时间和死亡数 ,所有动物用药后观察 15d。同时收集各组动物治疗后的标本 ,进行病毒分离 ,按常规测定病毒滴度。另设病毒对照组、0 .9%NaCl对照组、阳性药阿昔洛韦对照组 (ACV)。结果　PCV对HSV感染鼠有保护作用 ,5 0mg·kg-1·d-1时 ,HSV 1和HSV 2感染鼠的存活率分别为 6 0 %和 75 % ,平均存活天数分别为 (11.3± 3.6 )和 (12 .9± 2 .8)d ,随着药物剂量增加 ,疗效增强 ,该药还能降低组织内病毒滴度 ,阻止体内病毒复制 ,抗HSV作用类似于相同剂量的ACV。OBJECTIVE: To study the therapeutic effect of penciclovir (PCV) on herpes simplex virus (HSV) infection. METHODS: Every mouse was injected intraperitoneally 0.03 mL HSV-1 or HSV-2 solution with the titer of 100 LD 50 0.1 mL-1. After 24 hours, the mice were treated by hypodermic PCV with the doses of 20, 50, 100, 250 mg&middotkg -1&middotd-1 respectively for 5 days. At the same time, the symptoms, survival rate and survival time of mice were observe. After the mice died, their organs were collected, then the virus was isolated and tested quantitatively. Negative and positive controls were also studied. RESULTS: After using PCV 50 mg &middotkg-1&middotd-1, the survival rates of the mice infected with HSV-1 or HSV-2 were 60% and 75%, respectively. The average survival time were (11.3±3.6) days and (12.9±2.8) days respectively. With the increase of the dose, the protection effect of PCV on the infected mice increased correspondingly. The titer and reproduction of virus were also reduced in vivo. When given in the same dose, PCV had the similar effect to ACV on HSV. CONCLUSION: PCV had protective effect on mice infected with HSV.

关 键 词：喷昔洛韦 单纯疱疹病毒感染 治疗 抗病毒作用 

分 类 号：R978.7[医药卫生—药品]