RAPD分析野生和养殖三角帆蚌的遗传多样性  

RAPD analysis of genetic diversity of wild and cultured Hyriopsis cumingii

作　　者：华丹[1] 顾若波[1] 白云飞[2] 闻海波[1] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心,江苏无锡214081 [2]东南大学分子与生物分子电子学教育部重点实验室,江苏南京210076

出　　处：《水产学报》2003年第6期540-544,共5页Journal of Fisheries of China

基　　金：国家科技基础工作项目(我国水产种质资源数据库及网络建设子专题)资助

摘　　要：用150个随机引物对野生三角帆蚌和养殖三角帆蚌进行随机扩增多态DNA(RAPD)分析,最终筛选出20个引物,分别能扩增出1～10条大小不等的片段,片段长度在500～2000bp之间。野生三角帆蚌和养殖三角帆蚌的种内相似系数分别为0.787和0.833,显示野生种群基因组发生的变异较养殖种群大。野生三角帆蚌和养殖三角帆蚌种群间遗传距离为0.054,表明三角帆蚌野生和养殖种群亲缘关系比较接近。The genetic diversity of wild and cultured Hyriopsis cumingii was analysed using the random amplified polymorphism DNA(RAPD) technique. 20 random primers were sieved from 150 random primers, which could amplify 1 to 10 fragments individually with the fragment length varying from 500 to 2 000 bp. The intra-population genetic similarity of wild and cultured Hyriopsis cumingii is 0.787 and 0.833, respectively. It shows that gene variability in wild population is greater than that in cultured population. The inter-population genetic distance between wild and cultured Hyriopsis cumingii is 0.054. This means two populations of wild and cultured Hyriopsis cumingii have close relationship.

关 键 词：RAPD分析 野生蚌 饲养管理 三角帆蚌 遗传多样性 

分 类 号：S966.22[农业科学—水产养殖]