低能离子注入在花生四烯酸(AA)高产菌株选育中的研究  

Breeding of arachidonic acid-producing strain by low-energy ion implantation

作　　者：袁成凌[1] 姚建铭[1] 王纪[1] 余增亮[1] 

机构地区：[1]中国科学院等离子体物理研究所离子束生物工程学重点实验室,合肥230031

出　　处：《辐射研究与辐射工艺学报》2003年第4期237-242,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家"九五";"十五"重点科技攻关项目支持;专题编号:96-538-01-02;2001BA302B-04

摘　　要：利用低能离子束生物技术对花生四烯酸产生菌(Mortierella alpina)进行诱变选育。研究表明,在离子注入(10keV,3×1014N+/cm2)条件下,后代菌株离散程度明显高于自然分离。经连续诱变处理,最终获得一株花生四烯酸高产菌I49-N18,该菌每升培养液可得生物量26.3g,干菌体中油脂含量为33.8％,其中花生四烯酸的含量占总脂的52.36％。而其AA产量高达4.66g/L,比对照N7菌株产量提高126.2％,且继代遗传功能稳定,表明I49-N18是一株极具工业化前景的高产菌,同时证明离子注入是一种有效的诱变手段。Low energy ion implantation technology was used in mutation breeding on arachidonic acid (5, 8, 11, 14-eicosatetraenoic acid, AA) yielding starting strain, Mortierelle alpina N7. The results indicate that dispersion of descendants of the N7 strain implanted with 10keV N+ (3XI014 N+/cm2) was bigger than that in natural division strain. I49-N18, a high-yield AA-producing strain, was screened out by continuous mutagenicity. The biomass, lipid in biomass, AA in lipids of the high-yield strain were 26.3g/L, 33.8%(w/w), and 52.36%(w/w), respectively. The AA content in culture was 4.66g/L, which is 126.2% higher than the control, and its descendiblity was stable. It is concluded that L49-N18 is a promising strain for industrialization, and that ion implantation has remarkable mutagenic effect on microorganism.

关 键 词：离子注入 花生四烯酸 诱变选育 菌株离散 高产菌 发酵 筛选 

分 类 号：S565.203.5[农业科学—作物学] Q547[生物学—生物化学]