中国汉族人群肺结核易感基因的病例对照研究  

A case-control study on VDR and NRAMP1 gene polymorphisms with susceptibility to pulmonary tuberculosis in Chinese Han population

作　　者：刘玮[1] 张翠英[2] 田磊[3] 李春芝 吴晓明[1] 赵秋敏[1] 张泮河[1] 杨淑梅 杨红[1] 曹务春[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071 [2]解放军第309医院,北京100091 [3]解放军第91医院,兖州272100 [4]解放军总参三部防疫队,北京100091

出　　处：《军事医学科学院院刊》2003年第6期409-412,451,共5页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助 ( 3 0 2 7114 4)

摘　　要：目的 :探讨VDR ,NRAMP1基因多态性与中国汉族人群肺结核发病间的关联。方法 :采用病例对照研究设计 ,用聚合酶链反应_限制性片段长度多态性分析 (PCR_RFLP)法检测VDR基因FoKⅠ和TaqⅠ位点 ,NRAMP1基因中INT4 ,D5 4 3N及 3'UTR位点的基因型别 ,并对肺结核相关危险因素进行问卷调查 ,应用SPSS软件进行单因素和多因素非条件Logistic回归分析。结果 :2 0 0 1年 4月到 2 0 0 2年 6月 ,共有 110名肺结核病例 [平均年龄 (2 7.6 5± 12 .72 )岁 ]和 180名对照 [平均年龄 (2 7.2 6± 9.2 1)岁 ]纳入本研究 ,单因素分析表明病例组中FoKⅠ_ff,D5 4 3NG A及 3′UTRTGTG + del基因型频率显著高于对照组 ,OR值 (95 %CI)分别为 3.36 (1.4 3～ 7.89) ,2 .2 2 (1.0 3～ 4 .78)和 1.93(1.14～ 3.2 6 ) ,TaqⅠ与INT4位点各基因型在病例和对照中的分布无显著性差异。多因素分析中调整了接触史和卡介苗接种史两个因素后 ,FoKⅠ_ff,D5 4 3NG A及 3′UTRTGTG + del基因型仍与肺结核发病显著性相关 ,调整OR值 (95 %CI)分别为 3.15 (1.0 6～ 9.33) ,3.0 4 (1.12～ 8.2 7)和 2 .36 (1.2 0～ 4 .6 4 ) ,而TaqⅠ和INT4位点多态性与肺结核发病仍无显著关联。结论 :中国汉族人群VDR_FoKⅠ ,NRAMP1_D5 4 3N和NRAMP1_3′UTR位点多态性可?Objective:To investigate the potential role of VDR and NRAMP1 gene polymorphisms in susceptibility to pulmonary tuberculosis in Chinese Han population.Methods:Case-control study design was adopted. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques were used to type VDR gene polymorphisms (TaqⅠ and FoKⅠ) and NRAMP1 gene polymorphisms (INT4,D543N and 3′UTR). Information on related risk factors of pulmonary tuberculosis was collected using a standard questionnaire. Univariate and multivariate unconditional logistic analyses were conducted using SPSS for window software.Results:A total of 110 well defined pulmonary tuberculosis patients and 180 unrelated normal controls were recruited for the study. All of them were male servicemen with mean age 27.65±12.72 and 27.26±9.21 years,respectively. The univariate analysis demonstrated that the FokⅠ-ff, D543N G/A and 3′UTR TGTG+/del genotypes occurred more frequently in patients, with OR (95%CI) being 3.36 (1.43-7.89), 2.22(1.03-4.78)and 1.93(1.14-3.26),respectively. After adjusting two related environmental factors (exposure history and BCG immunization) in the multivariate analysis, the FokⅠ-ff, D543N G/A and 3′UTR TGTG+/del genotypes were still significantly overrepresented in cases, with adjusted OR(95%CI) being 3.15(1.06-9.33), 3.04(1.12-8.27)and 2.36(1.20-4.64),respectively. However, no significant association was observed between INT4, TaqⅠ genetic polymorphisms and pulmonary tuberculosis.Conclusion:VDR and NRAMP1 genetic polymorphisms might be involved in the susceptibility to pulmonary tuberculosis among Chinese Han population.

关 键 词：肺结核 维生素D受体基因 自然抗性相关巨噬细胞蛋白1基因 病例对照研究 

分 类 号：R521[医药卫生—内科学]