板蓝根中靛蓝和靛玉红的提取及其质量分数的测定  

Extraction and determination of mass ratio of Indigo and Indirubin in Indigowoad root

作　　者：刘依[1,2] 韩鲁佳[1,2] 阎巧娟[1,2] 刘向阳[1,2] 

机构地区：[1]中国农业大学工学院 [2]现代精细农业系统集成研究教育部重点实验室,北京100083

出　　处：《中国农业大学学报》2003年第6期5-8,共4页Journal of China Agricultural University

基　　金：国家"九五"重点攻关专题

摘　　要：采用直接比色法测定板蓝根中靛蓝、靛玉红的质量分数。对靛蓝、靛玉红在 2 4 0～ 70 0nm波段进行扫描 ,确定其最大吸收波长分别为 6 0 1和 5 31nm。分别通过单因素试验和正交试验 ,确定提取靛蓝、靛玉红的最佳工艺参数。结果表明 ,乙醇体积分数为 75 %时 ,靛蓝和靛玉红的提取物得率最高 ;提取时间为 4h时 ,靛蓝和靛玉红的提取物得率最高。正交试验确定的最佳提取工艺参数为 :药材粒度 4 0目 ,提取时间 4h ,乙醇体积分数 75 % ,此时 ,提取物得率达 1 74 %。Extraction and determination of mass ratio of Indigo and Indirubin in Indigowoad root were studied. Indigo and Indirubin were scanned from 240 to 700*!nm. From the spectrogram we can see that the largest absorbing wavelength of Indigo and Indirubin was 601 and 531*!nm respectively. The best parameters of technology have been determined through single factor and orthogonal test on extracting indigo and Indirubin respectively, results showed that ethanol with a volume ratio of 75% was suitable solvent to extract Indigo and Indirubin and 4 hours was the proper time to extract Indigo and Indirubin. Through orthogonal test the best parameters of technology were obtained. The results are as the following: the herbal granularity is 40 eye, extraction time is 4*!h, ethanol with a volume ratio of 75%. On this condition, the extracting rate was up to 1.74%.

关 键 词：板蓝根 靛蓝 靛玉红 提取技术 质量分数 测定技术 单因素试验 正交试验 

分 类 号：R282.71[医药卫生—中药学]