毛细管电泳中使用二元环糊精体系拆分CBI-氨基酸对映体  

ENANTIOSEPARATION OF CBI-AMINO ACIDS BY CAPILLARY ELECTROPHORESIS WITH DUAL CYCLODEXTRIN SYSTEMS

作　　者：沈江珊[1] 赵书林[1] 李舒婷[1] 钟新仙[1] 

机构地区：[1]广西师范大学化学化工学院,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2003年第4期69-73,共5页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(20265001);广西自然科学基金(0236013)

摘　　要：在毛细管胶束电动色谱中,以β-环糊精(β-CD)和羧甲基-β-环糊精(CM-β-CD)组成的二元体系作为手性选择剂,对两种相对较难手性拆分的萘-2,3-二甲醛氨基酸衍生物(CBI-DL-Ser,CBI-DL-Glu)进行对映体分离。结果表明,该二元环糊精体系在拆分CBI-DL-Ser和CBI-DL-Glu时具有协同效应,在实验选定的条件下,两者均能达到完全手性拆分。Dual cyclodextrin systems of β-cyclodextrin (β-CD) and carboxymethyl-β-cyclodextrin (CM-β-CD) were tested as chiral selectors for the enantiomeric separation of amino acids derivatized with naphthalene-2, 3-dicarboxaldehyde (CBI-AAs) with micellar electrokinetic capillary chromatography. Results showed that the resolution and enantioselectivity of this approach are superior to either β-CD or CM-β-CD used alone. The optimum molar ratio of β-CD to CM-β-CD is 1:1 at a total concentration of 20 mmol/L. In this work, 2 CBI-DL-AAs (CBI-DL-Ser, CBI-DL-Glu) enantiomers were baseline resolved.

关 键 词：毛细管电泳 二元环糊精 CBI-氨基酸对映体 毛细管胶束电动色谱 协同效应 对映体分离 

分 类 号：O629.71[理学—有机化学] O657.8[理学—化学]