不同因素对牛-兔种间重构卵融合率的影响  

Effect of Some Factors on the Fusion Rate of Bovine-rabbit Interspecies Reconstructed Eggs

作　　者：章孝荣[1,2] 刘亚[3] 张运海[4] 陈大元[1] 廉莉[1] 李劲松[1] 金仁桃[3] 章志国[3] 章美玲[3] 汪存利[3] 

机构地区：[1]中国科学院动物研究所生殖生物学国家重点实验室 [2]安徽农业大学畜牧水产学院,合肥230036 [3]安徽农业大学畜牧水产学院 [4]中国农业大学生物学院

出　　处：《农业生物技术学报》2003年第6期620-623,共4页Journal of Agricultural Biotechnology

基　　金：中国科学院计划生育生殖生物学国家重点实验室客座课题;安徽省"十五"重大科技专项(01603006)。

摘　　要：研究了用不同方法处理的核供体细胞(休眠期和非休眠期的体细胞、4℃冷藏1～5 d 或12～14 d 的体细胞)、融合液中加入细胞松弛素 B(cytochalasin B,CB)以及卵供体兔龄等因素对牛-兔种间重构卵融合率的影响。结果发现,以休眠期或非休眠期的体细胞作核供体,其融合率和在融合过程中的死卵率均无显著差异(P>0.05);将核供体细胞(休眠期或非休眠期的体细胞)在4℃冷藏1～5 d或12～14 d,与新鲜的核供体细胞相比,对重构卵的融合率和在融合过程中的死卵率无显著影响(P>0.05);在融合液中加入 CB 后对融合率无明显影响(P>0.05),但在融合过程中死卵率显著升高(P<0.05);用青年兔(3～5月龄)的卵母细胞作受体与用经产兔(10～12月龄)的相比,其融合率无显著差异(P>0.05),但在融合过程中,用青年兔卵母细胞作受体的重组卵的死卵率极显著低于用经产兔卵作受体的重组卵(P<0.01)。The test was conducted to study some factors,including donor cell treated with different methods(Go-cells and non-Go-somatic cell,somatic cells refrigerated 1～5 d or 10～12 d at 4℃),fusion solution containing cytochalasin B and the age of rabbits(3～5 month young rabbits or 10～12 month multiparous rabbits)as oocyte donor,and influencing fusion rate of bovine-rabbit interspecies reconstructed eggs.The results showed that there were no significant differences(P>0.05)in fusion rate and mortality rate of eggs reconstructed with Go-cells or non-Go-cells as nuclear donor somatic cells,no significant effect(P>0.05)on fusion rate and mortality rate of the reconstructed eggs when nuclear donor somatic cells(Go-cells or non-Go-cells)were treated by refrigeration 1～5 d or 10～12 d at 4℃,the fusion rate of reconstructed eggs was not changed significantly(P>0.05)when adding cytochalasin B into fusion solution,but the mortality rate was increased significantly(P<0.05),and significant effect was not found(P>0.05)in fusion rate of the eggs reconstructed with young rabbit oocyte as recipient,but the mortality rate was significantly decreasing(P<0.01) compared with multiparous rabbit ones.

关 键 词：核供体细胞 重构卵 细胞松弛素B 融合率 

分 类 号：Q813.7[生物学—生物工程] S823[农业科学—畜牧学]