TLC法鉴定皮肤及肌肉组织中的青霉素  

Application of TLC in Identification of Penicillin-injected Skin and Muscular Tissues

作　　者：卢英强[1] 宣兆艳[1] 崔佰君[1] 徐晶华[1] 祁柏宇[1] 刘莉[1] 张林庆[2] 于广池[2] 

机构地区：[1]吉林大学基础医学院法医学教研室,吉林长春130021 [2]长春市公安局

出　　处：《吉林大学学报（医学版）》2003年第6期780-781,共2页Journal of Jilin University：Medicine Edition

摘　　要：目的 :建立一种快速鉴定动物及人皮肤或肌肉组织中青霉素的方法。方法 :利用薄层层析( TLC)法将动物模型及人体皮肤、肌肉组织中青霉素分离提纯后进行鉴定。结果 :从实验动物及人皮肤或肌肉组织中分离提纯青霉素获得的色斑与青霉素标准品色斑和 Rf值完全相同 ,层析色谱清晰 ,色斑鲜明 ,可在正常环境中保存 1 92 h以上。结论Objective:To establish a method to identify quickly if the skin and muscular tissue of animals and human beings was injected with penicillin. Methods:Penicillin was isolated and purified from skin and muscular tissue,then it was identifred by thin layer chromatography (TLC). Results:The spots and Rf ratios of the penicillin isolated and purified from skin or muscular tissue of experimental animals and human beings were the same with the standard penicillin mark.The chromatography was clear,the spots were distinct,and they could be preserved for more than 192 h in normal circumstances. Conclusion:TLC may provide sufficient scientific evidence for medicolegal expertise in medical tangles dealing with deaths from penicillin hypersensitivity.

关 键 词：色谱法 薄层 青霉素类 分离 提纯 副作用 药物过敏 法医学 司法鉴定 

分 类 号：R978.1[医药卫生—药品] D919.4[医药卫生—药学]