压电石英晶体微阵列免疫传感器检测IgG的实验研究  

Determination of human immunoglobulin G by piezoelectric quartz crystal microarray immunosensor

作　　者：姚春艳[1] 陈庆海[1] 陈斌[1] 府伟灵[1] 

机构地区：[1]第三军医大学西南医院检验科,重庆400038

出　　处：《重庆医学》2003年第12期1611-1613,共3页Chongqing medicine

基　　金：国家 8 63计划重大专项 (2 0 0 2AARZ2 0 2 3) ;国家"九五"科技攻关项目 (96 A2 3 0 4 0 4 ) ;国家自然科学基金资助项目 (30 2 70 388) ;军队"九五"课题 (98M0 94) ;军队"十五"课题 (0 1MA1 80 ;0 1L0 65) ;重庆市应用基础研究项目 (2 0 0 2 1 0 1

摘　　要：目的　构建一种新型压电石英晶体人免疫球蛋白 (IgG)的免疫微阵列传感器。方法　抗IgG单克隆抗体采用蛋白A(SPA)法固定在AT切向、基频 1 0MHz的石英晶体微阵列金膜电极表面制成抗体敏感膜 ,构成压电石英晶体IgG免疫微阵列传感器。结果　构建的IgG压电石英晶体免疫微阵列传感器对IgG的响应特性良好 ,其检测下线为 0 .5mIU/ml。 结论　研制的压电石英晶体IgG免疫微阵列传感器具有灵敏度高、特异性好、成本低廉、操作简单、快速 ,能在线实时检测等优点 ,可用于临床实验诊断。Objective To construct a new type of piezoelectric quartz crystal micro array immunosensor for the determination of human immunoglobulin G (IgG).Methods The crystal was 10MHz AT cut quartz with gold coated electrodes.The immobilization of the monoclonal anti body of IgG onto the surface of the gold electrodes was accomplished via protein A.Results Experiment results showed that the piezoelectric immunosensor had good response to IgG,the frequency shifts were linearly dependent on the IgG concentration in the range of 0 5mIU/ml.The analytical results from this approach were in satisfactory agreement with those of by fluorescent immunoassay( P >0.05).Conclusion Piezoelectric microarray immunosensor for determination of IgG is of high sensitivity,good specificity,low cost,simple operation,quickly and real time detection etc.It is suitable for detecting IgG in serum in clinical laboratory.

关 键 词：压电 石英晶体 传感器 免疫球蛋白IGG 

分 类 号：Q64[生物学—生物物理学]