按蚊中肠前酚氧化酶原与约氏疟原虫卵囊黑化的关系  

STUDY ON THE RELATIONSHIP BETWEEN PROPHENOLOXIDASE IN THE MIDGUT OF ANOPHELES MOSQUITOES AND MELANIZATION IN OOCYSTS OF PLASMODIUM YOELII

作　　者：邱宗文[1] 张锡林[1] 徐文岳[1] 

机构地区：[1]第三军医大学基础部病原生物学教研室,重庆400038

出　　处：《中国寄生虫病防治杂志》2003年第6期321-324,共4页Chinese Journal of Parasitic Disease Control

基　　金：国家自然科学基金资助项目 (No .30 2 0 0 2 37)

摘　　要：目的　分析约氏疟原虫卵囊黑化期间按蚊中肠内前酚氧化酶原 (Prophenoloxidase ,PPO)的变化 ,探讨中肠PPO与疟原虫卵囊黑化的关系。　方法　解剖分离约氏疟原虫感染前和感染后 3、5、7、11和 15d斯氏与大劣按蚊中肠 ,匀浆后经SDS PAGE和转膜 ,以抗烟草天蛾PPO的抗体进行免疫印迹分析 ,并于感染后第 5d开始在镜下观察约氏疟原虫卵囊黑化情况 ,直至第 15d止。　结果　斯氏与大劣按蚊中肠在约氏疟原虫感染前和感染后的不同时期经免疫印迹均可检测到 1条分子质量约为 67ku的PPO阳性蛋白带 ,但感染后的PPO蛋白带比感染前明显增强 ,而且同时间点大劣按蚊中肠PPO蛋白带比斯氏按蚊明显 ,尤其在感染的后期大劣按蚊中肠PPO蛋白带明显比斯氏按蚊增强 ;在感染后 7d可见大劣按蚊中肠内发育的约氏疟原虫卵囊部分黑化 ,在 15d时可见疟原虫卵囊完全黑化。　结论　按蚊中肠PPO含量增加 ,尤其是长时间内维持在较高水平与约氏疟原虫卵囊黑化密切相关。Objective To explore the relationship between the melanotic encapsulation of Plasmodium yoelii and prophenoloxidase of the midgut by analysis the changes of prophenoloxidase in the midgut of Anopheles mosquitoes during melanotic encapsulation of oocysts of P. yoelii . Methods Midguts were dissected out from both Anopheles stephensi and An. dirus before and after infection with P. yoelii at 3、5、7、11 and 15 day. The homogenated midguts were analysed by SDSK-PAGE and Western blotting using Manduca sexta prophenoloxidase IgG polyantibody. The melanotic encapsulation of oocysts of P. yoelii was observed by microscope from 5 to 15 day after infection. Results One prophenoloxidase band with about molecular weight of 67 ku was displayed from both An. stephensi and An. dirus whether at pre-infection or at different days of post-infection with P. yoelii by Western blotting technique, and the prophenoloxidase bands were obviously enhanced after infection. However, the prophenoloxidase bands of midgut in the An. dirus were much more visible than those in the An. stephensi at the same time point after infection. Partial and complete melanotic encapsulation of P. yoelii in the midgut of An. dirus were observed from 7 to 15 day after infection. Conclusion The increasing content of prophenoloxidase in the midgut of Anopheles mosquitoes and keeping it for a long specially maybe have close correlation with the melanotic encapsulation of P. yoelii .

关 键 词：约氏疟原虫 斯氏按蚊 大劣按蚊 中肠 前酚氧化酶原 PPO 卵囊黑化 免疫学 

分 类 号：R531.3[医药卫生—内科学]