同种肝细胞脾内移植治疗大鼠肝衰竭的实验研究  

Hepatocyte transplantation intrasplenically for rats with acute hepatic failure

作　　者：滕光菊[1] 白雪帆[1] 黄长形[1] 孙永涛[1] 徐哲[1] 聂青和[1] 刘节[2] 顾炳权[3] 

机构地区：[1]中国人民解放军第四军医大学唐都医院全军感染病诊疗中心,陕西省西安市710038 [2]中国人民解放军第四军医大学唐都医院病理科,陕西省西安市710038 [3]中国人民解放军第四军医大学唐都医院检验科,陕西省西安市710038

出　　处：《世界华人消化杂志》2003年第12期1915-1918,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨经脾内同种异体移植培养的原代肝细胞与肝细胞悬液治疗大鼠药物性肝衰竭的疗效,并观察脾内移植肝细胞的生物学特性.方法:采用D-氨基半乳糖(D-gal)建立大鼠急性肝衰竭模型,24h后随机分为3组进行治疗,Ⅰ组:经脾内移植体外培养3d的肝细胞2×107;Ⅱ组:经脾内移植预孵育8h的肝细胞悬液2×107;Ⅲ组:经脾内注射生理盐水1 mL.观察受体大鼠的存活率、肝脏功能和病理变化及移植肝细胞的生物学特性.结果:Ⅰ组、Ⅱ组大鼠存活率(78%,71%)与Ⅲ组大鼠存活率(23%)相比具有显著性差异(P＜0.01,P＜0.05),肝功能各项指标均有明显改善,肝组织病理改变较轻.而Ⅰ,Ⅱ组大鼠的存活率、肝功能改变方面,没有统计学差异.经HE和PAS染色证实,移植的肝细胞在受体脾内结构和功能保持较好.结论:经脾内移植的培养肝细胞和肝细胞悬液均能提高大鼠药物性肝衰竭的存活率、改善肝功能及肝脏组织病理变化.而培养肝细胞与细胞悬液相比,统计学上没有明显差异,但结果显示培养肝细胞仍具有一定的优越性.AIM:To study the effects of intrasplenic transplantation of primary cultured hepatocytes and isolated hepatocytes on rats with acute hepatic failure(AHF)induced by D-galac- tosamine(D-gal),and to observe the biological character- istics of transplanted hepatocytes in the spleen. METHODS:AHF rats were induced by D-gal.Hepatocyte transplantation was carried out 24 hours after intoxication, and the rats were divided into three groups randomly.In Group Ⅰ,rats received intrasplenic injection of 2×10~7 hepato- cytes cultured for 3 days,whereas in Group Ⅱ,rats received 2×10~7 hepatocytes incubated for 8 hours intrasplenically. In Group Ⅲ,rats received 1mL normal saline intrasplenically as control.Then the survival rates,liver function,liver his- tology and the biological characteristics of transplanted hepatocytes in all rats were observed. RESULTS:Compared with Group Ⅲ rats,a significant im- provement in survival rates was observed in Group Ⅰ and Ⅱ (78% vs 23%,P<0.01 and 71% vs 23%,P<0.05 respectively).Three days after hepatocyte transplantation, the liver functions in Groups Ⅰ and Ⅱ rats were restored significantly compared with those of Group Ⅲ,and the liver pathologic changes were mild. But no difference was found in the survival rates and liver functions between Groups Ⅰ and Ⅱ.By the HE and PAS staining,our data indicated that the structure and function of hepatocytes transplanted kept basically well. CONCLUSION:Intrasplenic transplantation of primary cul- tured hepatocytes and isolated hepatocytes can prolong the survival of AHF rats and improve the liver function and histology,and no statistically significant differences are observed between Groups Ⅰ and Ⅱ.Our data suggest that hepatocytes cultured for 3 days may be an alterative for hepatocyte transplantation.

关 键 词：同种肝细胞脾内移植 大鼠 实验研究 原代肝细胞 肝细胞悬液 药物性肝衰竭 生物学特性 

分 类 号：R657.3[医药卫生—外科学]