氟、砷及氟砷联合经口染毒对大鼠肝UDS细胞影响的研究  

Studies on the UDS of Liver Cells in Rats Fed with Fluoride,Arsenic and Fluoride Combined with Arsenic in Drinking Water

作　　者：邢绥光[1] 买尔江[1] 玛依拉[1] 王惠民[1] 顾丹天[1] 王国荃[1] 肖碧玉[1] 马龙[1] 张晨[1] 

机构地区：[1]新疆医学院组织胚胎学教研室,新疆医学院环境卫生学教研室

出　　处：《劳动医学》1995年第4期11-13,36,共4页

摘　　要：本文用程序外ＤＮＡ合成（ＵＤＳ）试验方法检测氟、砷及氟砷联合染毒对大鼠肝脏ＤＮＡ的损伤及修复的影响。将雄性Ｗｉｓｔａｒ大鼠随机分为四组：氟组、砷组，氟砷联合组及对照组。分别饮用含氟化钠１２５ｐｐｍ、三氧化二砷７５ｐｐｍ、氟化钠１２５ｐｐｍ加三氧化二砷７５ｐｐｍ的水及蒸馏水。结果染毒３个月的大鼠肝脏，在氟组其ｃＰｍ均值为１５８０．７±１９６，明显高于对照组３７７±３４。砷组的ｃＰｍ均值为２３９．３±１０４，低于对照组。氟砷联合组的ｃＰｍ均值为３０９．３±５３，接近于但略低于对照组。染毒６个月脾大鼠肝脏，氟、砷及氟砷联合组的ｃＰｍ均值均低于对照组，其掺入百分率分别为对照组的４８．３％（氟组）、３８％（砷组）、８７％（氟砷联合组）。此结果说明慢性氟、砷及氟砷联合中毒可以引起大鼠肝细胞的ＤＮＡ损伤，并影响其修复损伤的能力。另外，本实验的结果还表明氟与砷有拮抗作用。Unscheduled DNA synthesis was measured by scintillation counter in primary cultures of rat hepatocytes.Rats were fed with sodium fluoride(Na F),trivalent arsenic trioxide(As2O3)and NaF combined with As_2O_3,respectively,indrinking water. Treatment with the NaF for three months resultec in the induction of UDS.The average value of ￣3H thymidine incorporation in NaF group was 1580.7±197cpm,It was significantly greater than that in control group(377±34cpm).In As2O3 group the average value(239.3±104cpm)was lower than that in control group.However.in combined NaF with As_2O_3 group the cpm value (309.3±53)was closed to that in control group .Exposure of rats to the chemicals for six months caused a lower￣3H thymidine incorporation in all three treated groups than that in control group.The percentage of cpm in NaF,As2O3 and combined group was 48.3%,38% and 87% in turn.These results indicated that 6-month exposure of rats to NaF,As_2O_3 or combined NaF with As_2O_3 could cause DNA damage and inhibit DNA repair.In addition,these results suggested that fluoride and arsenic could be antagonist.

关 键 词：氟中毒 砷中毒 大鼠 肝UDS细胞 DNA损伤 流行病学 

分 类 号：R595[医药卫生—内科学]