Studies　of　the　Expression　of　Estrogen　Receptor　Gene　in　the　Rat　Uterus　during　the　Estrous　Cycle　and　Periimplantational　Period  

作　　者：张沅 秦武轩 赵炳顺 范植明 邹继超 张永莲 

机构地区：[1]StateKeyLaboratoryofMolecularBiology,ShanghaiInstituteofBiochemistry,AcademiaSinica,Shanghai200031,China [2]StateKeyLaboratoryofReproductiveBiology,BeijingInstituteofZoology,AcademiaSinica,Beijing100080,China

出　　处：《Journal of Reproduction and Contraception》1995年第2期65-73,共9页生殖与避孕（英文版）

摘　　要：The correlation or serum estradiol concentrstion and uterine estrogen receptor (ER) gene expression (ERn and ERc quantitated by Dextrsn Coat Charcoal assay and ER mRNA by Northern blotting) was studied during the rat estrous cycle and early Pregnant stage (dl-d10). The ER gone expression was up - regulated by estrogen and the levels of ER mRNA synchronized with the changes of ER protein, suggesting that estrogen influenced the trsnscriPtional step of the ER gene. Post-coitum ER expression increased with the serum estrsdiol progressively, reached a peak on d4-ds (Just before implantation), but drastically dropped to the nadir on d6-d7 (during implantation) and then recovered. It was of interest to discover that ER mRNA level in the nonimplantstion sites (NIS) of uterus was much higher than that in the implantstion sites (IS).The correlation or serum estradiol concentrstion and uterine estrogen receptor (ER) gene expression (ERn and ERc quantitated by Dextrsn Coat Charcoal assay and ER mRNA by Northern blotting) was studied during the rat estrous cycle and early Pregnant stage (dl-d10). The ER gone expression was up - regulated by estrogen and the levels of ER mRNA synchronized with the changes of ER protein, suggesting that estrogen influenced the trsnscriPtional step of the ER gene. Post-coitum ER expression increased with the serum estrsdiol progressively, reached a peak on d4-ds (Just before implantation), but drastically dropped to the nadir on d6-d7 (during implantation) and then recovered. It was of interest to discover that ER mRNA level in the nonimplantstion sites (NIS) of uterus was much higher than that in the implantstion sites (IS).

关 键 词：IMPLANTATION Estradiol (E_2) Estrogen receptor (ER) gene mRNA 

分 类 号：R339.22[医药卫生—人体生理学]