溶胶-凝胶固定酪氨酸酶电极的研究  

Study of the Sol-gel Amperometric Biosensor Based on Tyrosinase

作　　者：刘俊女[1] 崔莉凤[1] 

机构地区：[1]北京工商大学,北京100037

出　　处：《化学传感器》2003年第4期53-60,共8页Chemical Sensors

摘　　要：研制了用溶胶-凝胶包埋的测酚酪氨酸酶碳糊电极。研究以四乙氧基硅烷(TEOS)为前驱体制备SiO_2溶胶-凝胶材料的机理,利用红外光谱法对材料进行了结构表征,确定了制备溶胶-凝胶和制作酶电极的最优化条件。所研制的溶胶-凝胶酪氨酸酶碳糊电极的工作条件为:工作电位-100mV(vs,SCE)、工作pH值5.40、测量时间3min,电极对苯酚的检测下限为1.00×10^(-6)mol/L,线性区间为1.00×10^(-6)mol/L～1.00×10^(-4)mol/L。相对标准偏差RSD和相对误差RE分别达1.04％和0.002％,此电极对邻甲酚、对苯二酚、邻苯二酚、对氯苯酚都有良好的响应,对邻氨基酚、间苯二酚、对甲苯酚、邻硝基酚、2.4二甲基酚响应不好。有机干扰物苯、甲苯、丙酮、乙酸乙酯、抗坏血酸对它无影响。电极在使用72h后,电极响应最好,一周后电极仍可用于检测。A amperometric tyrosinase carbon paste electrode was developed for detection of phenols based on sol - gel materials. The mechanism for preparing the sol - gel materials by using the hydrolysis and polycondensation of telramethoxysilicane TEOS was studied, The formation process of the material were studied by means of IR spectrometry. The optimum conditions for constructing the tyrosinase electrode by using sel - get matrix were established. The working potential of the tyrosinase electrode based on sol-gel materials was -100 mV(vs, SCE), the working pH was 5. 40, the time of measurement was 3 min. The detection limit was 1. 00× 10-6 mol/L, the linear range were 1. 00×10-6 ~ 1. 00×10~4 mol/L. The RSD was 1. 04% and the RE was 0. 002%. 0 - cresol, hydroquinone, catechol, p - chlorophenol had good response on the electrode, and o - amninoanisole, resorcinol, p - cresol, o - nitrophenol, 2, 4 - dinitrophenol had bad response on the electrode. Organic interfereces such as benzene, toluene acetone, ascorbic acid had no effect on the electrode. The response of the electrode was best after using the electrode for 72 hours and it can be used to detect phenol after one week.

关 键 词：溶胶 凝胶 酪氨酸酶电极 固定 四乙氧基硅烷 苯酚 生物传感器 

分 类 号：TH83[机械工程—仪器科学与技术]