日本血吸虫信号蛋白14-3-3的虫体免疫定位  

Immunolocalization of the Signaling Protein l4-3-3 of Schistosoma japonicum

作　　者：刘庆中[1] 沈继龙[2] 汪学龙[2] 

机构地区：[1]温州医学院附属第一医院检验科,温州,325000 [2]安徽医科大学病原生物学教研室,安徽省基因研究重点实验室,合肥,230032

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第6期330-332,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金 (No:30 1 70 84);安徽省自然科学基金 (No :0 0 4 4 5 4 7)资助项目~~

摘　　要：目的　研究抗重组日本血吸虫信号蛋白 1 4 3 3(rSj1 4 3 3)单克隆抗体对天然Sjl4 3 3的结合活性 ,观察Sj1 4 3 3在虫体内的定位。　方法　从阳性钉螺体内逸出尾蚴 ,感染家兔 ,分别于感染后 1 5d和 4 2d剖杀 ,静脉灌注法收集童虫和成虫制备冰冻切片。利用rSj1 4 3 3单克隆抗体 ,间接免疫荧光法探讨信号蛋白 1 4 3 3虫体内的分布。　结果　免疫荧光染色结果显示 ,rSj1 4 3 3单克隆抗体可特异性地结合天然Sj1 4 3 3抗原表位 ,背景清晰 ,Sj1 4 3 3广泛分布在雌、雄成虫的皮层、皮下层、肌层和实质层 ,前三种组织中特异性荧光呈线状分布 ,实质中特异性荧光弥散 ;童虫中Sj1 4 3 3也广泛的分布在皮层、皮下层、肌层和实质层。　结论　免疫荧光染色成功地确定了Sj1 4 3 3蛋白在成虫和 1Objective To study the localization of the signaling protein 14-3-3 of Schistosoma japonicum (Sj14-3-3)in the parasite. Methods Cercariae were collected from the infected Oncomelania hupensis for the infection of rabbits. Fifteen-day-old schistosomula and adult worms obtained from infected rabbits 15 and 42 days post-infection were used for frozen sections and indirect immunofluorescence staining with monoclonal antibody to rSj14-3-3. Results The results showed that the Sj14-3-3 distributed mainly in the tegument, subtegument, muscle, and parenchyma of both adult worms and 15-day-old schistosomula. Conclusion The wide distribution and large sites of Sj14-3-3 in the parasite were clearly demonstrated, which established a significant clue for further studies of biologic actions and application of 14-3-3 protein.

关 键 词：日本血吸虫信号蛋白14-3-3 虫体 免疫定位 单克隆抗体 SJ14-3-3 成虫 童虫 

分 类 号：R383.24[医药卫生—医学寄生虫学] R392[医药卫生—基础医学]