泡球蚴感染BALB/c小鼠IgG亚类和细胞因子的动态观察  

Kinetic Analysis of Cytokines and Immunoglobulin G Subclass in BALB/c Mice Infected with Echinococcus alveolaris

作　　者：李富荣 石佑恩[2] 史大中[3] D.A.Vuitton P.S.Craig 

机构地区：[1]深圳市人民医院 [2]华中科技大学同济医学院寄生虫学教研室,武汉43000 [3]兰州医学院寄生虫学教研室,兰州730000 [4]法国贝尚松大学中心医院 [5]英国苏尔沃德大学生命科学院

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第6期357-360,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：欧共体基金资助项目 (STD 2 )~~

摘　　要：目的　观察小鼠感染泡球蚴后其体液免疫的动态变化。　方法　BALB/c小鼠感染泡球蚴后 ,分别于 2、4、8、1 2、1 6、2 0及 2 5wk(IgG含量达高峰 )取脾脏制备淋巴细胞悬液体外培养 ,分别以多房棘球蚴抗原 (EmAg)、伴刀豆球蛋白A (ConA)刺激诱生可溶性白细胞介素 2受体 (IL 2R)、肿瘤坏死因子α(TNF α)及白细胞介素 1 (IL 1 ) ;以植物凝集素(PHA)刺激诱生干扰素γ(IFN γ)。检测培养上清中IL 2R、TNF α、IL 1及IFN γ含量。各组均设RPMI1 6 40培养液平行对照。检测血清中一氧化氮 (NO)及特异性免疫球蛋白G(IgG)亚类水平。　结果　小鼠感染泡球蚴 1 6wk后NO水平明显升高 ,IgG、IgG1和IgG3水平升高 ,IgG2a及IgG2b呈低水平。感染后的前 1 2wk ,脾淋巴细胞以分泌IL 2R和TNF α为主 ,1 2wk后以IFN γ为主、1 6wk后以IL 1为主。　结论　小鼠感染泡球蚴后的前 8wk呈Th1反应。Objective To observe the dynamic change of immune response in mice infected with Echinococcus alveolaris(AE) at difference period of time, and to explore hostalveo’s immune regulation. Methods The infection lasted and was followed up for 25 weeks. The spleen cells from BALB/c mice infected with AE stimulated with EmAg and ConA or PHA in vitro . IL-2R, IFN-γ, TNF-α, IL-1 and specific IgG subclasses were determined by ELISA. NO was tested by chemical assay. Results NO level sharply rised in 16 weeks after BALB/c mice were infected with AE. The levels of IgG1 and IgG3 significantly increased 8 weeks after infection, and remained elevating throughout the period of observation. IgG3 showed slight increase, IgG2a and IgG2b appeared low level following infection. The production of IL-2R and TNFα increased significantly 8 weeks of infection, while IL-2R sharply decreased in 12 weeks of infection. During the period of 2-12 weeks of infection there was an increase in IL-1 secreting. The level of IL-1 and TNFα rapidly increased since 16 weeks post infection. High level of IFN-γ was detected during the period of observation, and showed a peak at 12 weeks. Conclusion Th1 is the major response in the early stage of infection,which is replaced by Th2 response in later period of infection.

关 键 词：泡球蚴感染 BALB/C小鼠 IGG亚类 细胞因子 可溶性白细胞介素2受体 肿瘤坏死因子α 白细胞介素1 

分 类 号：R532.32[医药卫生—内科学] R383.33[医药卫生—临床医学]