钩棘单睾吸虫形态学观察  

Morphological Observation of Haplorchis pumilio

作　　者：林金祥 陈宝建 朱凯 程由注 李友松 许龙善 张榕燕 

机构地区：[1]福建省寄生虫病研究所,福州350001

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第6期361-362,共2页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的　对钩棘单睾吸虫 (Haplorchispumilio)进行形态学观察 ,为与其相似吸虫的鉴别提供依据。　方法家猫感染钩棘单睾吸虫囊蚴 4 5d后解剖 ,从小肠粘膜收集成虫 ,压片、染色、观察。收集虫卵、囊蚴、后尾蚴 ,观察其形态、结构等。检查麦穗鱼不同部位肌肉 ,分析确定钩棘单睾吸虫囊蚴寄生部位。　结果　成虫腹吸盘已退化 ,只有生殖吸盘 ,其盘上有锯齿状小钩 4 4～ 4 8枚。虫卵大小平均 31 .2× 1 6 .7μm ,壳光滑 ,肩峰不明显。囊蚴直径平均为 1 6 8.5μm ,囊内幼虫布满鳞状体棘。囊蚴只寄生在鱼鳍基部与鱼体连接的肌肉内。后尾蚴 ,大小平均为 4 45× 95μm ,全身披鳞状体棘 ,生殖吸盘上的小钩大部分可见。　结论　囊蚴形态特征、寄生部位和成虫有无生殖吸盘及盘上的小钩数和形状 。Objective To provide the basis for the differentiation with similar species of intestinal flukes through observing the figure of Haplorchis pumilio. Methods Adults of H. pumilio were collected from the intestine of the cat which was infected with the encysted cercariae of H.pumilio for 45 days. The worms were observed after staining. Eggs and metacercariae of H.pumilio were collected and examined for their shape, size and morphological characteristics. Pseudorasbora parva, the fish host, was examined for the parasitized sites of metacercariae. Results The principal characteristics of the adults is the acetabulum degradation. There are only the genital sucker with 44-48 hamuli. The average measurement of eggs is 31.2×16.7 μm with a smooth shell. Its aceromion is not evident. The average diameter of metacercariae is 168.5 μm. There are squamous spines on metacercaria. The metacercariae only parasitize in the muscle between the basis of the fin and the fish body. The average measurement of metacercaria cyst is 445×95μm, with squamous spines on the body surface. Hamuli are found on the genital sucker of metacercaria cyst. Conclusion The morphological figures and parasitic sites of metacercaria, the genital sucker of the adult, and the number and form of the hamulus on the genital sucker provide basis for distinguishing H. pumilio from other intestinal flukes.

关 键 词：钩棘单睾吸虫 形态学 鉴别 寄生虫病 囊蚴 后尾蚴 

分 类 号：R383.2[医药卫生—医学寄生虫学] R532.2[医药卫生—基础医学]