广西人群转化生长因子β1基因869T/C(Leu10Pro)遗传多态性的研究  

A study on genetic polymorphism of TGF-β1 gene 869T / C( Leu10Pro) of Guangxi crowd

作　　者：黄华佗 向阳[1] 罗宏成[1] 陈健明[1] 吴成将 韦叶生[1] 

机构地区：[1]右江民族医学院附属医院检验科,百色533000

出　　处：《右江医学》2015年第5期529-533,共5页Chinese Youjiang Medical Journal

基　　金：国家自然科学基金(81260234;81560552);广西自然科学基金(2011GXNSFA018198);广西卫生厅重点科研课题(重2012086)

摘　　要：目的研究转化生长因子β1(TGF-β1)基因869T/C(Leu10Pro)位点多态性各等位基因及基因型在广西人群中的分布频率,对比不同种族之间的分布差异。方法采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术和DNA测序法检测210例广西人群TGF-β1基因869T/C(Leu10Pro)位点多态性,并结合相关文献,比较其在不同种族间的分布差异。结果广西人群TGF-β1基因869T/C(Leu10Pro)位点表现为TT、TC、CC三种基因型,频率分别为25.2%、50.5%和24.3%。T、C等位基因频率分别为50.5%和49.5%。其基因型和等位基因频率在不同性别间比较,差异无统计学意义(P>0.05),但与埃及人和英国人比较,差异均有统计学意义(P<0.05)。结论在广西人群中存在TGF-β1基因869T/C(Leu10Pro)位点多态性,其基因多态性的分布频率在不同性别间比较差异不显著,但与其他种族人群比较存在显著差异。Objective To study allele and genotype frequencies in polymorphisms of TGF-β1 gene 869T/C( Leu10Pro)in Guangxi crowd,so as to analyze the distributions of it among different ethnic groups. Methods The TGF-β1 gene 869 T / C( Leu10Pro) polymorphisms of 210 people in Guangxi were examined by polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP) method and DNA sequencing. And distribution disparity between different ethnic groups were compared combing with related documents. Results The TGF-β1 gene 869 T / C( Leu10Pro) showed three genotypes: TT,TC and CC,and the frequencies were 25. 2%,50. 5% and 24. 3%,respectively. Allele frequencies of T and C were 50. 5% and49. 5%,respectively. The allele frequencies and genotype distribution of TGF-β1 gene 869 T / C( Leu10Pro) had no statistically significant difference between men and women( P > 0. 05),but difference was statistically significant when comparing with the Egyptians and the British( P < 0. 05). Conclusion There are TGF-β1 gene 869 T / C( Leu10Pro) polymorphisms in Guangxi crowd,and difference of its distributions is not significant between men and women,but the difference is significant when comparing with other races.

关 键 词：转化生长因子Β1 基因多态性 种族 

分 类 号：R394[医药卫生—医学遗传学]