芜青花叶病毒外壳蛋白基因的克隆和表达  

CLONING AND EXPRESSION OF COAT PROTEIN GENE OF TURNIP MOSAIC VIRUS

作　　者：邱并生[1] 王晋芳[1] 王小凤[1] 沈学军 田波[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《微生物学报》1992年第2期145-147,共3页Acta Microbiologica Sinica

摘　　要：芜青花叶病毒(Turnip Mosaic Virus,Tu-MV)属于马铃薯 Y 病毒组,其基因组为单链RNA,寄主范围广,能侵染20个科的双子叶植物。在我国 TuMV 的分布遍及各地。Turnip mosaic virus(TuMV),a me-mber of the potyvirus group,and consi-dered to be the main virus that causesevere disease of crucifer plants,suchas Brassica Campestris,B.Pekinensis,Raphanus Sativus etc.The RNA wasextracted from TuMV,purified by dif-ferential centrifugation and CsSO_4 de-nsity gradient centrifugation,using oli-go-dT as a primer to synthesize cDNA,which was then cloned into vector pla-smid pUC19.It has been shown byimmunological screening and WesternBlot analysis that the No.30 cloneexpressed the coat protein gene product.The sequence analysis of coat proteingene is in prograss.

关 键 词：芜青花叶病毒 外壳蛋白基因 

分 类 号：S432.41[农业科学—植物病理学]