金合欢根瘤菌的几种酶活特性  

SEVERAL KINDS OF ENZYMATIC ACTIVITY CHARACTERISTICS OF RHIZOBIUM ISOLATED FROM ACACIA FARNESIANA

作　　者：靖元孝[1] 莫熙穆[1] 程双奇[1] 

机构地区：[1]华南师范大学生物系,广州510631

出　　处：《微生物学通报》1992年第1期9-12,共4页Microbiology China

摘　　要：金合欢根瘤菌Rhizobium sp.(Acacia farnesiana)的氧化酶、过氧化氢酶、脲酶、青霉素酶和β-半乳糖苷酶均呈阳性。经过酶活性定量测定表明金合欢根瘤菌有葡萄糖酸-6-磷酸脱氢酶(6PGD)和β-半乳糖苷酶的酶活性,而慢生型大豆根瘤菌未测到上述两种酶活性。根据聚丙烯酰胺凝胶电泳酯酶图谱,6株金合欢根瘤菌可分成两群:酋株AF1、AF2和AF3的酯酶图谱中有A带,AF4、AF5和AF6的酯酶图谱中没有A带。Rhizobium strains isolated from Acacia farnesiana were positive for oxidase, catalase, urease, penicillinase and β-galactosidase. The quantitative determination of enzymatic acti- vity indicated that these strains possessed 6-phosphogluconate dehydrogenase (6PGD) and β- galactosidase activities but Bradyrhizobium japonicum didn't possess 6PGD and β-galactosi- dase activities. Six strains isolated from A. farnesiana may be divided into two groups according to esterase pattern on PAGE: strains AF 1, AF2 and AF3' possessed A band on esterase pattern but strains AF4, AF5 and AF6 didn't.

关 键 词：金合欢根瘤菌 6PGD Β-半乳糖苷酶 

分 类 号：Q939.114[生物学—微生物学]