骨髓中表达白细胞介素5受体mRNA的CD_(34)^+细胞与支气管哮喘气道炎症的关系  

Bone marrow-derived CD_(34)^+ cells expressing interleukin-5 receptor messenger RNA and asthmatic airway inflammation

作　　者：毛辉[1] 王曾礼[1] 刘春涛[1] 

机构地区：[1]四川大学华西医院呼吸内科,成都610041

出　　处：《中华结核和呼吸杂志》2003年第3期152-156,共5页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金资助项目 (39770 34 0 )

摘　　要：目的　探讨支气管哮喘 (简称哮喘 )小鼠骨髓 (BM)中表达CD+ 34 与白细胞介素 5受体(IL 5RmRNA+ )的造血细胞 (CD+ 34 IL 5RmRNA+ 细胞 )在气道炎症中的作用。方法　以卵白蛋白(OVA)及生理盐水致敏并激发Balb/c小鼠 ,建立各哮喘及对照组 (A组 )模型。分别于OVA及生理盐水首次激发后 1、6、12、2 4、48h处死小鼠 ,取支气管肺泡灌洗液 (BALF)、外周血 (PB)及BM标本备用。测定BALF中嗜酸性粒细胞 (EOS)、PB中有核细胞及EOS计数及BM中有核细胞数 ;用流式细胞仪分别测定PB及BM中CD+ 34 细胞占相应有核细胞的比例并推算其相对计数 ;用免疫组化结合原位杂交法分别标记骨髓细胞CD+ 34 抗原及IL 5RmRNA ,定位BM中CD+ 34 IL 5RmRNA+ 细胞并计数其占CD+ 34 细胞的比例。结果　 (1)OVA激发后 6h组 ,BALF中EOS计数为 (2 67± 1 0 0 )× 10 5/L ,与A组 [(0 46±0 0 6)× 10 5/L]比较差异有显著性 (P <0 0 1) ;OVA激发后 12h组 ,BALF中EOS、PB中EOS计数分别为 (7 74± 1 98)× 10 5/L、(2 91± 0 64 )× 10 8/L ,与A组 [(0 46± 0 0 6)× 10 5/L、(1 43± 0 3 7)× 10 8/L]比较 ,差异有显著性 (P均 <0 0 1) ;OVA激发后 2 4h组 ,BALF中EOS、PB中EOS计数分别为 (19 43±3 69)× 10 5/L、(3 93± 0 5 1)×Objective To study the possible role of bone marrow derived hematopoietic cells expressing CD + 34 and IL 5 receptor messenger RNA (IL 5R mRNA +) in asthmatic airway inflammation Methods Balb/c mice were sensitized and challenged by ovalbumin (OVA) to establish the asthmatic model The control mice were sensitized and exposed to sterile saline The mice were killed at different time points after challenged by OVA and sterile saline, and bronchoalveolar lavage fluid (BALF), peripheral blood (PB) and bone marrow (BM) were prepared Eosinophils (EOS) in PB and BALF, and nuclear cells in PB and BM were counted The percentage of CD + 34 cells to nuclear cells in PB and BM, and the relative number of CD + 34 cells in PB and BM were calculated by flow cytometry, Immunocytochemistry and in situ hybridization were used to observe the hematopoietic cells with co localized expression of CD + 34 and IL 5R mRNA (CD + 34 IL 5R mRNA +) cells in BM, and the percentage of CD + 34 IL 5R mRNA + to CD + 34 cells was calculated Results (1) At 6h after OVA challenge, the number of EOS in BALF[(2 67±1 00)× 10 5/L] was significantly increased as compared with the number in controls [(0 46± 0 06)×10 5/L]( P <0 01) At 12h after OVA challenge, the numbers EOS in BALF [(7 74±1 98)× 10 5/L] and in PB[(2 91±0 64)×10 8/L] were significantly higher than those in the controls( P <0 01) At 24h after OVA challenge, the numbers of EOS in BALF [(19 43±3 69)×10 5/L] , in PB [(3 93±0 51)×10 8/L] and the percentage of CD + 34 IL 5R mRNA +cells to CD + 34 cells [(301±90)‰ ] in BM were increased to the highest levels The differences were significant as compared with the corresponding parameters in the controls ( P <0 01). At 48h after OVA challenge the numbers of EOS [ (12 05±5 31)×10 5/L] in BALF and the percentage of CD + 34 IL 5R mRNA + cells in BM[ (221± 53)‰] were decreased, but were st

关 键 词：骨髓 白细胞介素5受体 基因表达 CD34^+细胞 支气管哮喘 气道炎症 

分 类 号：R562.25[医药卫生—呼吸系统]