靶向血管内皮生长因子受体2超声分子成像在小鼠原位胶质瘤血管新生和边界识别中的应用  

Application of vascular endothelial growth factor receptor 2-targeted ultrasound molecular imaging in intraoperative assessment of tumor angiogenesis and boundary identification in vivo

作　　者：张海娴[1] 李一鸣[1] 汪晓虹[1] 邵洁[1] 王怡[1] 

机构地区：[1]复旦大学附属华山医院超声医学科,上海200040

出　　处：《肿瘤影像学》2016年第3期248-251,共4页Oncoradiology

摘　　要：目的:探讨携带抗血管内皮生长因子受体2(vascular endothelial growth factor receptor 2,VEGFR2)单克隆抗体的超声微泡造影剂(microbubble,MB)在评价小鼠原位胶质瘤血管新生和边界识别中的作用。方法:以生物素-亲和素桥接法构建靶向微泡(MBv),体外鉴定其性质。建立小鼠GL261胶质瘤模型,分别注射MBv和普通微泡(MBc)进行超声造影检查。结果:成功构建偶联抗小鼠VEGFR2单克隆抗体的MBv,造影结果表明MBv较MBc能更好地评价小鼠胶质瘤血管新生和肿瘤边界。结论:MBv是良好的肿瘤靶向造影剂,应用MBv可较好地实现术中评价小鼠胶质瘤血管新生,更好地辅助术中超声导航。Objective: To develop and validate a targeted ultrasonographic(US) imaging agent with microbubbles(MBs) that binds to vascular endothelial growth factor receptor 2(VEGFR2) in a murine model for tumor angiogenesis. Methods: Targeted US imaging agent was prepared by bridged avidin biotin through binding anti-VEGFR2 antibody to the shell of perfluorocarbonfilled MBs. The binding specificities of targeted MB(MBv) and with nonlabeled MB(MBc) were tested with VEGFR2-positive cells(human umbilical vein endothelial cells, HUVECs). In vivo imaging signals of contrast-enhanced US by using anti-VEGFR2-targeted MBv and MBc were quantified in 10 mice bearing GL261 cells. Results: Attachment of HUVECs was significantly higher for MBv(mean MBs per cell 9.5±0.5) than MBc(mean MBs per cell 1.5±0.3). The imaging signal in the murine tumor angiogenesis model was significantly higher for MBv than for MBc. Conclusion: Targeted contrast-enhanced US directed at VEGFR2 improves in vivo visualization of tumor angiogenesis and boundary identification in a murine model of orthotopic gliomas.

关 键 词：血管内皮生长因子受体2 原位胶质瘤 超声 

分 类 号：R445.1[医药卫生—影像医学与核医学] R739.41[医药卫生—诊断学]