盐酸埃他卡林对小鼠脑组织缺氧的保护作用  

Protection of iptakalim hydrochloride against brain anoxia in mice

作　　者：刘蔚[1] 龙超良[1] 崔文玉[1] 汪海[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国临床药理学与治疗学》2004年第1期67-69,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：军队医药卫生科研基金"十五"重点项目 (№ 0 1Z0 2 5 )

摘　　要：目的 :研究盐酸埃他卡林 (Ipt)对脑组织缺氧的保护作用。方法 :小鼠给予Ipt 0 .5 ,2 .0 ,8.0mg·kg-13个剂量 (n =12 ,ig) ,连续给药 3wk ,并设生理盐水组和维生素E组作为对照 ,采用断头制备脑缺氧模型。活性采用化学比色法测定脑组织过氧化脂质(MDA)含量、谷胱甘肽过氧化物酶 (GSH PX)和超氧化物歧化酶 (SOD)活性。外周红细胞膜脂流动性以DPH为荧光探针 ,采用荧光偏振法测定。结果 :Ipt0 .5 ,2 .0 ,8.0mg·kg-13个剂量组延长小鼠断头后喘息持续时间 ,增加外周红细胞膜脂流动性 ,降低微粘度 ,且呈现量效关系。相同条件下 ,Ipt对MDA含量 ,GSH PX活性及SOD活性的影响不明显。结论 :Ipt对脑组织缺氧有显著保护作用 ,其作用可能与其改变血液流变学特性有关。AIM: To study the protection of iptakalim hydrochloride (Ipt) against brain anoxia in mice. METHODS: The models of brain anoxia were made in mice after the administration of Ipt ( 0.5 , 2.0 , 8.0 mg·kg -1 , n=12, ig)for 3 wk. NS and vitamin E were set as controls. The glutathione proxidase (GSH PX) activity, superoxide dismutase (SOD) activity and the lipid peroxide (MDA) content were determined in the brain homogenates of models, and the values of erythrocyte membrane fluidity were measured by the fluorescence polarization technique with DPH fluorescence probe. RESULTS: Compared with the control groups, Ipt ( 0.5 , 2.0 , and 8.0 mg·kg -1 , ig) markedly prolonged the breathing time and increased the fluidity of erythrocyte membrane in a dwse dependent manner, but had no apparent effects on the activity of GSH PX and SOD. CONCLUSION: The protection of Ipt against brain anoxia is related to increasing the fluidity of erythrocyte membrane and decreasing blood viscosity.

关 键 词：关键词 盐酸埃他卡林 脑缺氧 红细胞膜脂流动性 微粘度 过氧化脂质 超氧化物歧化酶 谷胱甘肽过氧化物酶 

分 类 号：R972.4[医药卫生—药品] R965[医药卫生—药学]