氯沙坦与替米沙坦对糖尿病大鼠心肌纤维化的实验研究  

Effect of Losartan and Telmisartan in reducing myocardial fibrosis in diabetic rats

作　　者：曹新营[1] 王志军[1] 黄宇玲[1] 刁增利[1] 杨文琦[1] 刘宁[1] 

机构地区：[1]河北联合大学附属医院心内科,河北唐山063000

出　　处：《慢性病学杂志》2015年第6期619-622,共4页Chronic Pathematology Journal

摘　　要：目的研究不同血管紧张素受体阻滞剂(ARBs)对糖尿病心肌纤维化的影响。方法将50只SD大鼠随机分为正常组(n=10)和糖尿病组(n=40),糖尿病组使用腹腔注射链脲佐菌素(STZ)方法建立糖尿病大鼠模型。将建模成功后36只大鼠随机分为三组:糖尿病模型组(10只)、氯沙坦治疗组(13只)和替米沙坦治疗组(13只)。采用免疫组化法检测各组大鼠心肌组织Ⅰ、Ⅲ型胶原、转化生长因子β1(TGF-β1)、结缔组织生长因子(CTGF)表达。计算每组的心脏指数和左心室指数;采取放免法测定心肌组织AngⅡ的含量。各组数据以均数±标准差(x±s)表示,组间比较采用方差分析、t检验,P<0.05为差异有统计学意义。结果糖尿病大鼠模型组的心脏指数、左心室指数、Ⅰ型胶原和Ⅲ型胶原、AngⅡ、TGF-β1、CTGF均分别显著高于正常组的(均P<0.05);与糖尿病模型组相比,氯沙坦治疗组和替米沙坦治疗组上述各指标均有明显降低(均P<0.05),但氯沙坦治疗组和替米沙坦治疗组之间差异均无统计学意义(P>0.05)。结论氯沙坦、替米沙坦均可明显抑制糖尿病心肌纤维化的发病过程。Objective To research the effect and the mechanism of different Angiotensin receptor blockers(ARBs) in improving myocardial interstitial fibrosis of diabetic rats.Methods The 50 male SD rats were randomly divided into the normal(n=10) and diabetic group(n=40).Rats of the diabetic group were injected intraperitoneally with streptozotocin(STZ) to be induced into diabetic rat models.In total,36 diabetic rats were induced.The 36 rats were divided into control(n=10),Losartan-(n=13) and Telmisartan-treated group(n=13).Expression of type Ⅰ and Ⅲ collagen,transforming growth factor β1(TGF-β1),connective tissue growth factor(CTGF) in myocardium were detected using immunohistochemistry technique.The cardiac index and left ventricle index were monitored.The content of angiotensin Ⅱ was detected using radioimmunoassay.Results The cardiac index,ventricle index,type Ⅰ collagen,type Ⅲ collagen,angiotensin Ⅱ,TGF-β1 and CTGF were significantly higher in the control group of the diabetic rats than those in the normal group(P<0.05).Compared with the control group of diabetic models,all these indexes were dramatically decreased in the Losartan or Telmisartan treated groups(P<0.05).There was no significant difference between the Losartan and Telmisartan treated groups(P>0.05).Conclusions Losartan and Telmisartan significantly restrained the myocardial interstitial fibrosis of diabetic rats.

关 键 词：氯沙坦 替米沙坦 糖尿病心肌病 心肌纤维化 

分 类 号：R587.2[医药卫生—内分泌] R542.2[医药卫生—内科学]