榉属植物总DNA提取方法研究  

Study on DNA extraction of Zelkova

作　　者：金晓玲[1] 乌云塔娜[2] 张智俊[2] 何平[2] 

机构地区：[1]浙江师范大学生命与环境科学学院,金华321004 [2]中南林学院经济林育种与栽培国家林业局重点实验室,株洲412006

出　　处：《植物研究》2004年第1期107-110,共4页Bulletin of Botanical Research

基　　金：国家林业局高技术项目 ( 99 14 )

摘　　要：榉属植物体内酚类、多糖等次生物质含量较高 ,严重影响从中提取总DNA的产量和质量。针对这一问题探索出一种适合榉属植物总DNA的提取方法 ,并对提取的总DNA进行了纯度和浓度的鉴定。结果表明此方法可有效去除次生物质对DNA的干扰 ,样品DNA的质量和纯度较高 。The content of phenolic compound, polysaccharides and other secondary metabolites is high in ligneous plant, which influences frequently the yield and quality of genome DNA when being extracted. In this study, a new method was introduced which do best for characterization the purity and concentration of genome DNA. The result showed that it could effectively eliminate the affections of the secondary materials to extracted genome DNA from Zelkova, and which be suitable for RAPD analysis and other geneticanalysis.

关 键 词：榉属 DNA提取 RAPD 木本植物 榆科 

分 类 号：Q933[生物学—微生物学] Q949.737.2