离子交换层析法提取黏杆菌素的研究  

Study on the process of extracting colistin by ion exchange chromatography

作　　者：邢维玲[1] 周希贵[1] 王贺祥[1] 宋渊[1] 章红[1] 

机构地区：[1]中国农业大学生物学院微生物系,北京海淀100094

出　　处：《中国兽医杂志》2003年第11期44-46,共3页Chinese Journal of Veterinary Medicine

摘　　要：本文采用 D15 2型弱酸性阳离子交换树脂对多黏类芽孢杆菌 (Paenibacillus polymyxa)发酵液中黏杆菌类(Colistin)的提取条件进行了研究。通过静态与动态吸附相结合的方法 ,确定了影响提取效果的三个重要参数即洗脱液 HCl浓度、上样液浓度和洗脱液流速。结果表明 ,当上样液浓度为 2 0 0 0 0 IU / ml,1m ol/ L HCl以 2 .0 ml/ min的流速洗脱时可获得最好的提取效果 ,收率达到 5 2 .8%。In this paper,the conditions of extracting colistin from the fermentative liquid by using of weak acidic cation exchange resin D152 were studied.Better conditions of extracting,sample consistency is 20 000 U/ml, 1 M HCl as eluant and the elution rate 2 ml/min,were found by the static and dynamic state experiments.Under this condition,colistin yielded at a rate of 52.8%.

关 键 词：离子交换层析法 提取技术 黏杆菌素 杀菌作用 饲料利用率 

分 类 号：S816[农业科学—饲料科学]