昆明山海棠碱对Jurkat淋巴瘤细胞株的细胞周期和凋亡时相的影响  

Effects of Tripterygium Hypoglaucum (Levl) Hutch alkaloids on cell cycle and time-dependent apoptosis of Jurkat T lymphoma cells

作　　者：杨录军[1] 敖琳[1] Nuesse Michael 徐颖[3] 曹佳[1] 

机构地区：[1]第三军医大学预防医学系卫生毒理学教研室 [2]GSF-National Research Center for Environment and Health,Neuherberg,Germany,D85764 [3]第三军医大学附属新桥医院临床药理基地

出　　处：《第三军医大学学报》2003年第17期1505-1507,共3页Journal of Third Military Medical University

基　　金：德国联邦教育科学研究技术部 (BMBF)基金资助项目 ;国家自然科学基金资助项目 (C30 10 0 2 4 1) ;第三军医大学中青年科研基金资助项目 ( 2 0 0 0 2 0 0 1)

摘　　要：目的　探讨昆明山海棠 (THH)碱对JurkatT淋巴瘤细胞的细胞毒性、细胞周期和凋亡时相特异性的影响 ,以了解THH碱诱导细胞凋亡的机制。方法　台盼蓝染色法检测细胞生长和细胞活力 ;DNA染色法、TUNEL标记结合流式细胞术检测细胞周期和凋亡时相特异性。结果　THH碱能有效抑制Jurkat细胞增殖、细胞活力下降 ,细胞在增殖周期中被阻滞于G1 期。THH碱首先能诱导S、G2 M期细胞凋亡 ,同时能诱导G1 期细胞凋亡。结论　提示THH碱能抑制Jurkat细胞的DNA合成 ,抑制细胞增增殖 ,并可显著诱导各期相细胞发生凋亡。Objective To study the effects of the cytotoxicity, cell cycle and time dependent apoptosis of Jurkat T lymphoma cells induced by Tripterygium Hypoglaucum (Levl) Hutch (THH) alkaloids so ad to explore the mechanisms of the apoptosis induced by the THH alkaloids. Methods Cell vitality and cell proliferation were measured by Typan blue staining. Cell cycle and the time dependent apoptosis were determined by DNA staining, TUNEL labeling and flow cytometry. Results THH alkaloids could effectively inhibit cell proliferation of Jurkat cells and induce the G 1 arrest and could induce apoptosis in G 2/S phase first and then G 1 phase. Conclusion THH alkaloids can inhibit DNA synthesis, cell proliferation and can also induce apoptosis of Jurkat T lymphoma cells in all phases.

关 键 词：昆明山海棠 细胞凋亡 G1期阻滞 细胞增殖 TUNEL 

分 类 号：R282.71[医药卫生—中药学] R730.21[医药卫生—中医学]