昆明山海棠碱通过线粒体途径诱导Jurkat淋巴瘤细胞株细胞凋亡  

Tripterygium Hypoglaucum (Levl) Hutch alkaloids induce the apoptosis of T lymphoma cells via the mitochondrial pathway

作　　者：杨录军[1] Nuesse Michael 余厚勇[1] 敖琳[1] 曹佳[1] 

机构地区：[1]第三军医大学预防医学系卫生毒理学教研室 [2]GSF-National Research Center for Environment and Health,Neuherberg,Germany,D85764

出　　处：《第三军医大学学报》2003年第17期1511-1514,共4页Journal of Third Military Medical University

基　　金：德国联邦教育科学研究技术部 (BMBF)基金资助项目 ;国家自然科学基金资助项目 (C30 10 0 2 4 1) ;第三军医大学中青年科研基金资助项目 ( 2 0 0 0 2 0 0 1)

摘　　要：目的　研究昆明山海棠碱通过线粒体途径诱导细胞凋亡的作用。方法　THH碱多剂量多时间点诱导Jurkat细胞后 ,采用Westernblotting检测细胞内Bcl 2表达的含量、采用JC 1原位染色方法、细胞免疫化学和流式细胞术等手段在单细胞水平分析细胞线粒体膜Apo2 .7分子以及线粒体膜电位 (ΔΨm)的变化。结果　THH碱诱导后Bcl 2蛋白表达下调 ,线粒体膜电位明显下降 ,线粒体膜Apo2 .7蛋白分子表达显著增加。结论　THH碱可通过线粒体途径诱导JurkatT淋巴瘤细胞株发生凋亡 ,并且BclObjective To explore the effects of Tripterygium Hypoglaucum (Levl) Hutch (THH) alkaloids on the apoptosis of Jurkat T lymphoma cells via the mitochondrial pathway. Methods After Jurkat cells were induced by THH alkaloids at different doses and at different time points, Bcl 2 expression was detected by Western blotting. Apo2.7 molecules on mitochondrial membrane and mitochondrial membrane potential (ΔΨm) were measured by JC 1 staining, immunocytochemistry and flow cytometry. Results THH alkaloids could effectively reduce mitochondrial membrane potential and Bcl 2 expression and increase Apo2.7 molecule expression in Jurkat cells. Conclusion THH alkaloids can induce the apoptosis of T lymphoma cells via the mitochondrial pathway. Bcl 2 takes part in the role of the starting of apoptotic function of mitochondria.

关 键 词：昆明山海棠 细胞凋亡 线粒体膜电位 Bcl—2 APO2.7 流式细胞术 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]