THH碱不通过钙离子信号通道诱导Jurkat淋巴瘤细胞株凋亡  

Role of [Ca^(2+)]i in the apoptosis of Jurkat T lymphoma cells induced by Tripterygium Hypoglaucum (Levl) Hutch alkaloids

作　　者：杨录军[1] Grundler Werner Nuesse Michael 敖琳[1] 曹佳[1] 

机构地区：[1]第三军医大学预防医学系卫生毒理学教研室 [2]GSF-National Research Center for Environment and Health,Neuherberg,Germany,D85764

出　　处：《第三军医大学学报》2003年第17期1518-1520,共3页Journal of Third Military Medical University

基　　金：德国联邦教育科学研究技术部 (BMBF)基金资助项目 ;国家自然科学基金资助项目 (C30 10 0 2 4 1) ;第三军医大学中青年科研基金资助项目 ( 2 0 0 0 2 0 0 1)

摘　　要：目的　探讨昆明山海棠碱在凋亡中对细胞内游离钙离子浓度的影响。方法　THH碱诱导Jurkat细胞后 ,采用Indo 1AM及PI进行细胞染色、流式细胞术等手段在单细胞水平上检测细胞内钙离子浓度的变化。结果　THH碱诱导后细胞内钙离子浓度未见明显变化。结论　THH碱不通过钙离子信号通道诱导Jurkat细胞凋亡。Objective To explore the roles of [Ca 2+ ]i in the apoptosis of Jurkat cells induced by Tripterygium Hypoglaucum (Levl) Hutch alkaloids. Methods After Jurkat cells were stained with Indo 1 AM and PI, changes of [Ca 2+ ]i were assessed by flow cytometry at the single cell level. Results THH alkaloids could induce no changes of [Ca 2+ ] inside cytoplasm. Conclusion THH alkaloids induce the apoptosis of Jurkat cells via other pathways rather than via the [Ca 2+ ] pathway.

关 键 词：昆明山海棠碱 细胞凋亡 钙离子 流式细胞术 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]