hERR1对乳腺癌细胞生长活性及ER转录激活功能的影响  

The in vitro effects of hERR1 on the growth activity of breast cancer cell strain and ER transactivation function

作　　者：陈健[1] 陈敏[1] 陈彬[1] 周长保[1] 李渝萍[1] 彭家和[1] 李蓉芬[1] 钟小林[1] 周度金[1] 

机构地区：[1]第三军医大学基础医学部生物化学与分子生物学教研室,重庆400038

出　　处：《第三军医大学学报》2003年第17期1527-1530,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 0 70 359) ;国家自然科学基金两个基地建设资助项目 ( 9910 76 194 6 )

摘　　要：目的　构建hERR1高表达的乳腺癌细胞株 ,研究hERR1对乳腺癌细胞株生长活性的影响及其机制。方法　PCR构建hERR1真核表达载体 ,转染乳腺癌细胞株 ,G418筛选稳定表达细胞株 ;MTT法研究hERR1高表达株的生长速率 ;瞬时转染结合CAT活性分析 ,研究hERR1对ER转录功能的影响。结果　筛选了hERR1高表达乳腺癌细胞株 ;局部高表达的hERR1在体外抑制乳腺癌细胞株的生长 ;hERR1以剂量依赖方式抑制ER转录激活功能。结论　局部高表达hERR1可能通过与ER竞争结合ERE ,抑制ER的转录活化功能 。Objective To establish a cell line which can stably overexpress hERR1 and to observe the effects of hERR1 overexpression on breast cancer cells. Methods An eukaryotic expression plasmid for hERR1, used for the infection of MCF 7 breast cancer cell line, was constructed by PCR. Cell line that expresses hERR1 stably was screened with G418. The growth rate of hERR1 transfected breast cancer cells was determined by MTT. Co transfection and CAT analysis were employed to examine the effects of hERR1 overexpression on ER transactivation function. Results The constructed cell strain could stably overexpress hERR1. MCF 7 cells transfected with hERR1 grew slower than those without being transfected. hERR1 reduced ER transactivation activity in the transient transfection assay in a dose dependent manner. Conclusion Overexpression of hERR1 may inhibit the growth rate of ER positive MCF 7 cells by means of competing with ER for binding ERE and hence inhibit the transactivition function of ER in vitro .

关 键 词：hERR1 乳腺癌 基因表达调控 

分 类 号：R392.12[医药卫生—免疫学] R737.9[医药卫生—基础医学]