原发性高血压患者G蛋白β_3亚单位基因C825T多态性的研究  

G protein β_3 subunit C825T polymorphism and essential hypertension in Chinese

作　　者：王鸿懿[1] 孙宁玲[1] 高颖[2] 苟淑琴[3] 

机构地区：[1]北京大学人民医院心内科,北京100044 [2]石家庄铁路中心医院心内科 [3]北京大学人民医院放射免疫实验室,北京100044

出　　处：《北京大学学报（医学版）》2003年第4期423-425,共3页Journal of Peking University:Health Sciences

摘　　要：目的 :研究中国大陆高血压人群中G蛋白 β3 亚单位 (GNB3)基因C82 5T多态性的分布及与血浆肾素 血管紧张素系统 (RAS)活性的关系。方法 :确诊原发性高血压的患者 4 0 8例作为试验组 ,14 0例健康成年人作为正常对照组 ,6 1例有高血压家族史的健康成年人作为高血压家族史阳性正常对照组。C/T多态性测定采用PCR -RFLP方法。结果 :各组GNB382 5T等位基因频率为 4 5 .6 %～ 5 6 .4 % ,各基因型在原发性高血压患者与正常人间分布差异无显著性。TT型高血压患者有较高的醛固酮水平和较低的血浆血管紧张素转换酶活性。结论 :G蛋白β3 亚单位 82 5TT基因型的中国大陆人群原发性高血压患者有较高的醛固酮水平和较低的血浆血管紧张素转换酶活性。Objective:To study the relationship between G protein β 3 subunit ( GNB3 )C825T variant and plasma renin angiotensin system(RAS) activity in Chinese essential hypertensive patients. Methods: Case control method was used. For 408 essential hypertensive patients who were enrolled in the trial groups, we chose 140 normotensives as control group 1 and 61 health persons with hypertensive familial history as control group 2, respectively. PCR-RFLP method was used to measure the C/T polymorphism. The results were observed by agarose gel eletrophoresis. Results: The frequencies of 825T allele were 45.6% to 56.4% in the three groups. Neither GNB3 genotype distribution nor the frequency of T allele was associated with essential hypertension. But patients with TT genotype had higher aldosterone level and lower angiotensin converting enzyme (ACE) activity than patients with CC genotype. Conclusion:In Chinese, patients with TT genotype had higher aldosterone level and lower angiotensin converting enzyme (ACE) activity.

关 键 词：原发性高血压 G蛋白 β3亚单位基因 C825T 多态性 

分 类 号：R544.1[医药卫生—心血管疾病]