稀有金属甲氨喋呤配合物与小牛胸腺DNA相互作用的研究  

Mechanism of Interaction between Methotrexate Metal Complexe and Calf Thymus DNA

作　　者：卢继新[1] 张贵珠[2] 黄志娜[2] 黄欣[1] 

机构地区：[1]天津医科大学药学院,天津300203 [2]南开大学化学院,天津300071

出　　处：《稀有金属》2003年第6期747-751,共5页Chinese Journal of Rare Metals

基　　金：国家自然科学基金资助项目 ( 2 95 75 2 0 0 );天津医科大学自然科学基金资助课题

摘　　要：用溴化乙锭为探针研究了稀有金属甲氨喋呤 (methotrexateMTX)配合物与小牛胸腺DNA的作用机制 ,探讨了其作用模式 ,即甲氨喋呤与DNA是非嵌插结合 ,稀有金属甲氨喋呤配合物与DNA之间的作用为静电方式和一定的嵌入方式。并求得稀有金属甲氨喋呤配合物与DNA的结合常数。The mechanism of the interaction between methotrexate (MTX), metal (Me) complexes and calf thymus (CT) DNA was studied by using ethidium bromide (EB) as a fluorescence probe. The results from varied experiments such as ultraviolet spectra, fluorescence spectra, fluorescence quenching curve and scatchard equation and circular dichroism etc, support that intercalation and electrostatic binding are the two major modes for interaction between Me MTX complexs and CT. DNA. The absence of the intercalation binding between the MTX and CT DNA is proved. The binding constant of Me MTX complexs with CT DNA was found.

关 键 词：金属配合物 溴化乙锭 甲氨喋呤 DNA 

分 类 号：TQ460.72[医药卫生—药物分析学]