电针内关对内毒素休克大鼠模型肺组织中一氧化氮合酶活性的影响  

Influence of Electroacupuncturing at Neiguan(PC 6) on NOS Express of Injuried Lung in Endotoxin Shock Model Rat

作　　者：黎晖[1] 杜少辉[2] 李伊为[1] 陈东风[1] 

机构地区：[1]广州中医药大学解剖教研室,广东510405 [2]深圳市中医院,广东518000

出　　处：《上海针灸杂志》2003年第11期3-6,共4页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目 (39970 91 4 );广东省中医药管理局项目 (98447);深圳市科技局项目 (2 0 0 0 0 4 0 87)

摘　　要：目的　观察电针内关 ( PC 6)对内毒素休克时肺损伤的保护作用和肺一氧化氮合酶 ( NOS)表达的影响。方法　 SD大鼠尾静脉注射内毒素 ( LPS) 1.5 m g/kg、腹腔注射 D-氨基半乳糖 ( D-Gal N) 10 0 m g/kg造成内毒素休克模型 ,用电针 PC 6和氨基胍 ( A G)作对照干预处理 ,用免疫组化方法检测诱生型一氧化氮合酶 ( i NOS)、内皮型一氧化氮合酶 ( e NOS)在肺组织内的表达。结果　电针 PC6可以使肺内 i NOS表达减弱 ,e NOS表达增强 ,肺损伤减轻。结论　电针 PC6可以减轻内毒素休克造成的肺损伤 ,这种保护作用可能是通过调节肺组织Objective To observe the effect of electroacupuncturing at Neiguan(PC 6) on acute lung injury and lung NOS express in endotoxin shock rat. Methods The endotoxin shock animal model was induced by LPS and D GalN. The expression of iNOS and eNOS was detected by immunohistochemistry. Results Electro acupuncturing at PC 6 may inhibit the express of iNOS, but promote the express of eNOS. The blood pressure retrieving smoothly and stably following electroacupuncture treatment. Conclusion Electroacupuncturing at PC 6 may be anti injury to the lung of endotoxin shock rats via modulating NOS protein express.

关 键 词：电针 内关 内毒素休克 肺组织 一氧化氮合酶 活性 

分 类 号：R245.97[医药卫生—针灸推拿学]