大鼠肺内降钙素基因相关肽和5-羟色胺免疫反应细胞的加龄变化  

Pulmonary CGRP and 5-HT immunoreactive cells in developing rat lungs

作　　者：樊平[1] 刘晔[1] 康林[1] 张峰[2] 刘力斗[2] 崔慧先[2] 

机构地区：[1]河北医科大学解剖学教研室,河北石家庄050017 [2]河北医科大学第二医院外科,河北石家庄050017

出　　处：《第四军医大学学报》2003年第7期614-617,共4页Journal of the Fourth Military Medical University

基　　金：河北省自然科学基金 (30 1 359)

摘　　要：目的 :探讨大鼠肺内降钙素基因相关肽 (calcitoningene relatedpeptiole ,CGRP)和 5 羟色胺 (5 hydroxytryptamine ,5 HT)免疫反应细胞的个体发生及分布 ,揭示肺神经内分泌细胞的功能及其内分泌机制的形态学基础 .方法 :应用免疫组织化学方法、激光扫描共聚焦技术及计算机图像分析 .结果 :CGRP和 5 HT阳性细胞最早见于胚 16d肺组织中 ,随胎龄增大其数量增多且分布广泛 ,胚 2 0d达高峰 ,生后阳性细胞数量减少 [神经内分泌 (NE) / 1× 10 3上皮细胞 (BE) :3 82± 13vs 94± 17;3 87± 4 7vs88± 18;P <0 .0 1,以出生后 1mo为例 ].共聚焦显微镜结果也证实随胎龄增加 ,CGRP和 5 HT阳性细胞数量增加且细胞荧光强度增强 ,而生后强度减弱 (P <0 .0 1) .相邻切片法观察可见多数表达CGRP的阳性细胞也含有 5 HT免疫反应物 .结论 :肺内CGRP和 5 HT阳性细胞的随龄变化显示其在肺的发育、分化、成熟的变化过程中具有重要作用 ,出生前后其数量变化明显的现象 ,可能与此阶段肺功能的急剧改变有关 .CGRP和 5 HT的共存表明两者在功能上具有相关性 .AIM: The paper is to explore the development of calcitonin gene related peptide (CGRP) and serotonin (5 HT) immunoreactive cells in lung and reveal the mechanism of the endocrine system. METHODS: The lung tissue was studied with immunohistochemitry and laser scanning confocal microscopy. The results were dealed with computer image analysis. RESULTS: Cells containing CGRP and 5 HT firstly appeared in the 16 day fetuses, and increased in number as gestation progressed and reached its peak at 20 day fetuses. The number of CGRP and 5 HT positive cells declined in postnatal rat lungs(NE/per 10 000 BE: 382±13 vs 94±17; 387±47 vs 88±18; P <0.01, 1 mo for example; P <0.01). Adjacent sections immunostained with antibodies against CGRP and 5 HT proved the coexistence of CGRP and 5 HT in neuroendocrine cells. The data of laser scanning confocal microscopy showed that the fluorescence intensity became strong with the development of fetuses and weak in postnatal rat lungs. CONCLUSION: CGRP and 5 HT positive cells changed with the development of the lung and this result suggested that these cells played an important role in the development, differentiation and maturity of the lung. The rapid changes in number during the period of birth may relate with the sharp change in function of the lung. The result of adjacent sections method proved the coexistence of CGRP and 5 HT in neuroendocrine cells. This finding suggests the possibility of functional interaction of CGRP and 5 HT.

关 键 词：肺神经内分泌细胞 降钙素基因相关肽 5-羟色胺 免疫组织化学 激光扫描共聚焦显微镜 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]