有机锗多酸衍生物的抗肿瘤作用及其机制  

Study of Anti-tumor and Mechanism of Organogermanium Poly-derivatinmves

作　　者：张煜[1] 王宝贵[1] 张桂英[1] 董晓路[2] 

机构地区：[1]吉林大学公共卫生学院,吉林长春130021 [2]吉林大学第一医院干部病房,吉林长春130021

出　　处：《癌变．畸变．突变》2004年第1期39-42,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:对有机锗多酸衍生物进行体外毒性、抑瘤率和对瘤细胞周期影响的研究,为临床应用提供科学依据。材料与方法:采用MTT法检测有机锗多酸衍生物对FL、L929的体外毒性效应,对SMMC-7721、Hela、K562的体外抑瘤效应,流式细胞仪单荧光染色法检测有机锗多酸衍生物对SMMC-7721的细胞周期影响。结果:①有机锗多酸衍生物在160.00 μg/ml以下时对FL、L929没有任何毒副作用,甚至有促进细胞生长的作用,160.00-1 280.00μg/ml范围内,随剂量增大毒性也增强。②有机锗多酸衍生物对SMMC-7721、Hela、K562的体外抑瘤效应曲线均为双峰曲线,对SMMC-7721、K562体外抑瘤率的第一个峰值在2.50μg/ml时分别是12.6％、32.8％,对Hela体外抑瘤率的第一个峰值在5.00μg/ml时,是10.6％,之后随剂量增大而减少,到80.00-1 280.00 μg/ml范围内,随剂量增加抑瘤率升高,到1 280.00μg/ml时,对上述3种细胞的抑瘤率分别达到66％、59.9％、65.8％。③有机锗多酸衍生物对SMMC-7721在5.00和320.00 μg/ml时作用48 h,均能延长G0,/G1期,缩短S、G2期。结论:有机锗多酸衍生物在微量时,对SMMC-7721、Hela、K562的生长有抑制作用,对SMMC-7721细胞周期有影响。BACKGROUND & AIM: To study the cytotoxicity on normal cell , the inhibitive effect of Organogermanium Poly - derivatimves on cancer cell and the effect on cell cycle in vitro, and provide a basis to clinical usage. MATERIAL AND METHODS: The cytotoxicity on FL, L929 and the inhibitive rate of Organogermanium Poly - derivatimves on SMMC - 7721, Hela, K562 in vitro were onducted with the method of MTT; The effect of Organogermanium Poly - derivatimves on cell cycle was studied by the way of One - Fluorescence Dying with Flow Cytometer. RESULTS: ①When the dosage reached 160.00 μg/ml, Organogermanium Poly - derivatimves did not show any cytotoxicity on FL and L929, and even stimulated cell growth; When the dosage was 160.00-1280.00 μg/ml, bigger dose showed higher cytotoxicity. ② In vitro, the inhibitive rate curve of Organogermanium Poly - derivatimves on SMMC - 7721, Hela, K562 were all two - peak curve. When the dosage reached 2.50 μg/ml,the first peak of the curve, the inhibitive rate of Organogermanium Poly - derivatimves on SMMC - 7721, K562 were 12.6 % , 32.8 % respectively, and on Hela, When the dosage reached 5.00 μg/ml, inhibitive rate was 10.6 %, and decreased with higher dose. When the dosage was 80.00-1280.00 μg/ml, the inhibitive rate rised with higher dose. When the dosage reached 1 280.00 μg/ml, the inhibitive rates were 66.0 %,59.9 %,65.8 % respectively. ③When the dasage reached 5.00 μg/ml, 320.00 μg/ml, after 48 hours, Organogermanium Poly - deriva-timves could extend G0/G1 and shorten S, G2 of cell cycle on SMMC - 7721. CONCLUSION: At micro - dose, Organogermanium Poly - derivatimves showed obviously inhibiton to SMMC - 7721, Hela, K562 and effect to cell cycle on SMMC - 7221,have also observed.

关 键 词：有机锗 多酸衍生物 体外抑瘤率 细胞周期 

分 类 号：R979.1[医药卫生—药品] R994[医药卫生—药学]