正交实验法优选孔石莼(Ulvan Pertusa)多糖的提取工艺  

Optimum extraction of polysaccharides from Ulva pertusa established by ortho gonal test

作　　者：于鹏展[1] 张虹[1] 牛锡珍[1] 徐祖洪[1] 李智恩[1] 

机构地区：[1]中国科学院海洋研究所

出　　处：《中成药》2004年第1期16-18,共3页Chinese Traditional Patent Medicine

基　　金：中国科学院前沿;方向性创新项目与青岛市科委基金支持;编号分别为KZCX3 SW 2 1 5.0 2 2 kj hh 71

摘　　要：目的 :优选并确立孔石莼多糖的最佳提取工艺 ;检测多糖中中性糖的组分。方法 :以孔石莼多糖的溶出率及总糖含量为指标 ,采用正交实验法对提取过程中水用量 (A) ,提取温度 (B) ,提取时间 (C)及 pH值 (D) 4因素进行优选研究 ,并以优选出的提取方案做平行实验。薄层层析法检测多糖中主要中性糖成分。结果 :影响多糖溶出率的显著因素是提取温度与提取时间 ,而影响总糖含量的显著因素仅为提取温度。多糖中主要的中性糖包括鼠李糖 ,木糖和葡萄糖。结论 :以多糖溶出率及总糖含量为考察指标的最优提取工艺为A1B3C3D3 。AIM: To optimize and establish the extraction techn ology of water-soluble polysaccharides from Ulva pertusa. TLC was employed to determine the mainly neutral sugars in the Ulvan. METHODS: The orthogonal test was employed to test the effects of th e four factors including volume of water, temperature of extraction, time of ext raction and pH of water on the yield and total carbohydrate contents of water-s oluble polysaccharides from Ulva pertusa and optimize the extraction technol ogy. Five groups of parallel tests were carried out by the optimum parameters es tablished and the sugar constituents of products were analyzed by TLC. RESULTS: Temperature of extraction and time of extraction have sign ificant effects on the extraction yield of water-soluble polysaccharides from Ulva pertu sa, but, for total carbohydrate content, only temperature of extraction was a significant factor. The mainly neutral sugars were composed of rhamnose, xylose and glucose. CONCLUSION: The optimum extraction technology was A 1B 3C 3D 3 when the yield and total carbohydrate content were considered as preferential pa rameters.

关 键 词：绿藻 孔石莼 孔石莼多糖 薄层层析 正交实验 

分 类 号：R284.2[医药卫生—中药学]