体外培养小鼠生殖细胞的碱性磷酸酶活性研究  

Alkaline Phosphatase Activity of Cultured Mouse Germ Cells

作　　者：李莲军[1] 何志云[1] 龚伟[1] 苗永旺[1] 秦先玉[1] 黄淑芬[1] 阮承迈[2] 

机构地区：[1]云南农业大学动物科技学院,云南昆明650201 [2]解放军军需大学动物科技系,吉林长春130062

出　　处：《动物医学进展》2003年第3期75-77,共3页Progress In Veterinary Medicine

基　　金：国家自然科学基金资助 (3980 0 1 0 6)

摘　　要：研究小鼠生殖细胞体外培养时碱性磷酸酶 (AP)活性表达 ,为鉴别精原细胞提供依据。分别培养 2日龄 ,6日龄 ,12日龄仔鼠及成年鼠生精上皮细胞 2 4～ 4 8h,进行 Gomori- Takamat-su组化染色 ,观察各类细胞形态及染色特性。结果表明 ,体外培养 2 4～ 4 8h,管周细胞为铺展面积较大的不规则形状 ,细胞表达 AP活性。支持细胞 (Sertoli cell)为不规则形状 ,不表达 AP活性。生殖细胞均为圆球形。性原细胞和精原细胞均表达 AP活性 ,部分精母细胞也表达 AP活性。To study the alkaline phosphatase (AP) activities of cultured mouse germ cells provide bases for identification of cultured mouse spermatogonia. The seminiferous epithelial cells dissociated from mice at ages from newborn to adult were cultured for 24 48 hours, and the AP activities of them were examined histochemically. the Peritubular cells have a wide spread irregular outline and expressed AP activity. Sertoli cells took the shape of irregularity and expressed no AP activity. All germ cells are spherical in outline. Gonocytes and spermatogonia all expressed AP activities. Some of spermatocytes expressed AP activity. Mouse spermatogonia express AP activity. AP activity can be used as an additional marker for cultured mouse spermatogonia.

关 键 词：体外培养 小鼠 生殖细胞 碱性磷酸酶 酶活性 

分 类 号：Q954.43[生物学—动物学]