转PEPC基因水稻接种固氮菌的效应  

Effect of Nitrogen-fixing Bacteria on PEPC Transgenic Rice ( Oryza sativa L. )

作　　者：严建民[1] 闫春玲[2] 林敏[2] 张保明[2] 陈炳松[1] 焦德茂[3] 

机构地区：[1]江苏省农业科学院原子能农业利用研究所,江苏南京210014 [2]中国农业科学院原子能利用研究所,北京100094 [3]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014

出　　处：《江苏农业学报》2003年第2期65-69,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家973项目"杂交稻高光效生理育种途径"(J1998010100)

摘　　要：利用^(15)N-同位素技术研究转PEPC基因水稻及无PEPC基因水稻在接种不同固氮菌后的光合特性、产量性状和固氮能力的变化。结果表明:接种水稻联合固氮工程菌处理与未接种固氮菌处理相比,转PEPC基因水稻的PEPC活性、PS Ⅱ光化学效率(Fv/Fm)、净光合速率均明显提高,生物学产量和经济产量也相应增加了38.08％和26.86％;与无PEPC基因水稻相比,转PEPC基因水稻能明显提高水稻联合固氮工程菌的固氮效率。这种水稻光合与固氮相互促进的机理值得深入研究。The photosynthetic properties, yield character of PEPC transgenic rice inoculated nitrogen-fixing bacteria and nitrogen-fixing capacity of nitrogen-fixing bacteria inoculated in PEPC transgenic rice were studied by using l5 N-isotope tracer technique. The results showed that the PEPC activity, PS Ⅱ photochemical efficiency and net photosynthetic rate of PEPC transgenic rice inoculated nitrogen-fixing bacteria increased significantly under high light intensity . The dry matter of plant and grain yield of PEPC transgenic rice increased 38.08% and 26.86% under it was inoculated with nitrogen-fixing bacteria. Compared with no PEPC gene rice, the PEPC transgenic rice could enhance nitrogen-fixing capacity of nitrogen-fixing bacteria. The mechanism of acceleration between photosynthesis of PEPC transgenic rice and nitrogen fixation of nitrogen-fixing bacteria in PEPC transgenic rice need to be studied deep.

关 键 词：转PEPC基因水稻 接种 固氮菌 光合特性 产量性状 固氮能力 

分 类 号：S511[农业科学—作物学]