狼尾草属牧草资源的RAPD分析  

RAPD Analysis on Forage Resources in Pennisetum

作　　者：钟小仙[1] 顾洪如[1] 向阳海[2] 任丽娟[2] 

机构地区：[1]江苏省农业科学院畜牧研究所,江苏南京210014 [2]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014

出　　处：《江苏农业学报》2003年第2期109-113,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省应用基础研究项目(BJ2000042)

摘　　要：利用RAPD分子标记技术对83份狼尾草属(Pennisetum)牧草资源(其中7株为“23A”不育系中的可育株)进行遗传多态性聚类分析。结果表明:①共筛选200个随机引物,其中10个引物对所有供试材料都能扩增出清晰的多态性条带,且重复性好;②83份材料可分为5类,其中7株23A不育系里的可育株与23A单独聚成一类,初步确定其为不育系23A的育性逆转株;③引物S1038(TCGCGGAACC)能扩增出“杂交狼尾草”的特异指纹图谱标记,为牧草品种杂交狼尾草的保护提供了分子鉴定基础。76 forage resources in Pennisetum and 7 fertile plants from male sterile line '23A' were analyzed by RAPD. The results showd-.①All materials appeared clear polymorphic band by using 10 primers selected from 200 primers. ②83 resources were divided into 5 groups. Since cluster Ⅲ only include 7 fertile plants from male sterile line 23A and sterile line 23A. It was basically confirmed 7 fertile plants from male sterile line 23A come from fertility reversion. ③A DNA fingerprinting amplified with S1038 ( TCGGGGAAGG) was constructed and that might be used in ' Hybrid Napier grass' identification.

关 键 词：狼尾草属 牧草资源 RAPD分析 分子标记技术 

分 类 号：S544.9[农业科学—作物学]