内蒙古锡盟蒙族群体VWA、FGA、PENTAE基因座的多态性研究  

The study of genetic polymorphism of VWA、FGA、PENTA Eloci in xi'meng District Mongolia population of Inner Mongolia

作　　者：李东霞[1] 苏秀兰[1] 毕力夫[1] 

机构地区：[1]内蒙古医学院分子生物学研究中心,呼和浩特010059

出　　处：《中国优生与遗传杂志》2003年第6期19-22,共4页Chinese Journal of Birth Health & Heredity

基　　金：内蒙古自治区自然科学基金 ( 2 0 0 10 90 6-10 ;2 0 0 10 90 6-16)

摘　　要：目的　了解内蒙古锡盟地区蒙族人群VWA、FGA、PENTAE 3个STR位点的遗传多态性分布并与汉族群体作对照 ,获得相应多态位点的群体遗传学数据。方法　采用多重PCR扩增技术和聚丙烯酰胺凝胶电泳方法 ,对 2 6 3名蒙族个体进行调查。结果　VWA、FGA、PENTAE 3个位点分别检出 9、10、2 2个等位基因 ,多态性分布均符合Hardy -Weinberg平衡定律。杂合度 (H)分别为 0 .8115、0 .84 87、0 .914 6 ,多态信息量 (PIC)分别为 0 .782 5、0 .82 98、0 .90 6 4。结论　上述 3个位点均具有较高的杂合度和多态信息量 ,是较理想的遗传标记系统。同时表明蒙汉群体基因频率分布在VWA位点无显著性差异 ,而在FGA、PENTAE位点存在极显著性差异 ,为人类遗传学和民族学研究提供了依据。Objective: To study the distribution of genetic polymorphism of VWA?FGA?PENTA E loci in Mongolia population and compared with Han population, and to construct genetic database. Methods: Multiple PCR and polyacrylamide gel electrophoresis were used to investigate the polymorphism of 3 STR loci in 263 cases Mongolia population. Results: 9 ,10 ,22 alleles were observed at these 3 STR loci. The genotype distribution in Mongolia population was in accordance with Hardy-Weinberg equilibrium, the expected heterozygosities for 3 loci were 0.8115?0.8487?0.9146 respectively, the polymorphism information contents (PIC) were 0.7825? 0.8298? 0.9064 respectively. Conclusion: The results demonstrated these 3 STR loci can be used as genetic marks. There was not significant difference in VWA loci between Mongolia and Han population. However there were very significant differences in FGA?PENTA E loci between Mongolia and Han population. The study provides valuable data which are beneficial to understanding the population genetics and national science.

关 键 词：聚合酶链反应 短串联重复序列 遗传多态性 内蒙古 锡盟地区 蒙族群体 VWA FGA PENTAE 群体遗传学 基因座 

分 类 号：R346[医药卫生—基础医学]