c-jun反义基因转染对缺氧复合烧伤血清处理心肌细胞保护作用的分子机制研究  

Molecular Mechanism of c-jun Antisense Gene Transfection in Protecting Cardiomyocyte from Injury of Hypoxia and Burn Serum Treatment

作　　者：胡安根[1] 黄跃生[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,重庆400038

出　　处：《中国医师杂志》2003年第12期1596-1598,共3页Journal of Chinese Physician

基　　金：国家杰出青年科学基金项目 (30 1 2 50 4 0 ) ;国家重点基础研究发展规划项目 (G1 9990 542 0 2 ) ;军队"十五"指令性课题项目 (0 1L0 66) ;高等学校骨干教师资助计划项目 ;军队首批临床高新技术重大项目

摘　　要：目的　探讨c -jun反义基因转染对缺氧复合烧伤血清处理心肌细胞保护作用的分子机制。方法　烧伤血清采自3 0 %TBSAⅢ度烫伤Wistar大鼠 ;采用含 1%O2 的混和气体作为缺氧模型 ;采用基因重组技术构建c -jun反义基因重组体 ;由Lipo fectaminekit介导心肌细胞转染 ;缺氧复合烧伤血清处理后 12、2 4和 48h不同时相点采用Westernblot检测c -jun蛋白 ,PKCα和JNK的表达变化。结果　加入烧伤血清并造成缺氧的非转染心肌细胞 (非转染组 ) ,c -jun蛋白 ,PKCα和JNK均显著表达 ,2 4h达最高峰 ;转染c -jun反义基因重组体 (转染组 )后 ,c -jun蛋白 ,PKCα和JNK表达均明显下降。结论　cObjective To explore the molecular mechanism of c-jun antisense gene transfection in protecting cardiomyocytes from injury of hypoxia and burn serum treatment. Methods Burn sera were collected from wistar rat with 30% total body surface area(TBSA) Ⅲ degree burn injury, and the mixture gas containning 1% O 2 was used as hypoxia model. The c-jun antisense gene recombinant vector was constructed. Neonatal wistar rat cardiomyocytes cultured in vitro were treated with hypoxia and burn sera. c-jun antisense gene recombinant vector was transfected into the cultured cardiomyocytes. Expressions of c-jun, PKCα and JNK were detected with western blot in the transfected and non-transfected cardiomyocytes. Results Expression levels of c-jun, PKCα and JNK significantly increased in the non-transfected cardiomyocytes when treated by hypoxia and burn sera, up to maximum 24 hour after the treatment. Expression levels of c-jun, PKCα and JNK in the transfected cardiomyocytes decreased significantly compared with non-transfected cells. Conclusions The transfection of the c-jun antisense gene recombinant vector protected cardiomyocytes from injury of hypoxia and burn sera treatment via down-regulating PKCα and JNK expressions.

关 键 词：PKCΑ JNK 缺氧 烧伤 血清处理 c-jun反义基因 基因转染 PCDNA3.1(+) 动物实验 心肌细胞保护作用 分子机制 

分 类 号：R644[医药卫生—外科学] R394[医药卫生—临床医学]