水稻谷蛋白启动子驱动下的ipt基因在转基因烟草中的表达  

Ipt gene driven under a gluteline promoter of rice seed expressed in transgenic tobacco plants

作　　者：申佩弘[1] 乔越美[1] 黄胜[1] 武波[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005

出　　处：《广西植物》2004年第1期49-51,共3页Guihaia

基　　金：教育部高等学校青年骨干教师基金 ;广西十百千人才工程专项经费 (2 0 0 0 2 0 6 )

摘　　要：利用农杆菌系统介导 ,采用叶盘转化法 ,将在水稻谷蛋白启动子驱动下的外源ipt基因导入烟草植株中 ,经过抗生素筛选、PCR与测序分析检测出转基因植株。成熟的转基因烟草种子经过ELISA细胞分裂素试剂盒检测 ,发现iPAs含量为对照的 2 .43倍 ,此外 ,种子的重量也增加了 7.8%。An ipt gene driven under the gluteline promoter of rice seed was introduced into tobacco plants by A.tumefaciens mediated transformation. After selected using antibiotics, PCR and sequencing analysis confirmed that ipt gene has integrated into the genomes of the tobacco plants. Cytokinin level in seeds was determined by ELISA kit. The result showed that the iPAs level of transgenic seeds is about 2.43 fold than CK, at the same time, the weight of seed has increased by 7.8 percent.

关 键 词：水稻 谷蛋白启动子 ipf基因 转基因烟草 基因表达 

分 类 号：Q943.2[生物学—植物学] S572[农业科学—烟草工业]