针刺大鼠后三里穴脑fMRI成像的初步研究  

fMRI pilot study on rat brain function while puncturing the acupoint ST36(Housanli)

作　　者：金香兰[1] 尹岭[1] 刘买利[2] 张许[2] 吴义根[3] 姚斌[1] 朱克[4] 

机构地区：[1]解放军总医院神经信息中心,北京复兴路28号100853 [2]中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室 [3]中国科学院高能物理研究所 [4]解放军总医院神经病学研究所

出　　处：《中国康复医学杂志》2003年第12期715-718,共4页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(批准号30171135);国家科技部基础研究重大项目前期研究专项(项目编号2001CCA00700)

摘　　要：探讨针刺大鼠的功能性磁共振(fMRI)成像的动物实验方法。方法:利用4.7T磁共振对9例雄性SD大鼠行针刺右侧后三里穴及穴旁的fMRI成像,采用统计参数图(SPM)软件进行统计学处理。结果:针刺大鼠右侧后三里穴可以引起下丘脑外侧区、下丘脑前区、下丘脑腹内侧等脑区MRI信号增加(P=0.001);两组第Ⅰ、第Ⅱ感觉运动皮质和尾壳核的MRI信号均增加,穴位组在P=0.001水平检出,穴旁组则在P=0.01或者P=0.05水平检出。结论:针刺大鼠后三里穴可引起下丘脑外侧区、下丘脑前区、下丘脑腹内侧区和丘脑室旁核等脑区MRI信号增加。fMRI是在活体动物实验中研究针刺中枢机制的可行方法之一。Objective:To explore the experimental method in obtaining fMRI signal on puncturing rat.Method:Data on changes of fMRI signal were obtained in9male Sprague-Dawley rat with4.7T MR imager while puncturing the right ST36(Housanli)or nonmeridian point(lateral area of leg1cm from ST36).The statistical analysis was performed by statistical parametric mapping(SPM).Result:There was fMRI signal increase in lateral,anterior and ventromedial hypothalamic region,et al(P=0.001)while puncturing St.36,whereas.SensorimotorⅠ,Ⅱ,and caudate putamen in both group but P levels were different(puncturing St.36:P=0.001,nonmeridian point P=0.01or P=0.05).Conclusion:Acupuncture on the ST36can lead to fMRI signal increase in lateral,anterior and ventromedial hypothalamic region,et al.fMRI is one of the feasible methods on study of acupuncture neural mechanism in vital animal experiments.

关 键 词：后三里穴 脑fMRI成像 针灸疗法 动物实验 神经递质 方法学 中枢调节机制 

分 类 号：R445.2[医药卫生—影像医学与核医学]