转Bt基因抗虫棉室内鉴定技术研究  

Studying on Transgenic Bt Cotton Using Kanamycin as an Indirect Identification Technique Indoors

作　　者：朱加保[1] 杨可胜[1] 产焰坤[1] 刘方志[1] 江本利[1] 徐道清[1] 

机构地区：[1]安徽省农业科学院棉花研究所,安庆246003

出　　处：《中国农学通报》2003年第6期69-70,共2页Chinese Agricultural Science Bulletin

基　　金：安徽省财政厅世行2期加灌科研项目资助

摘　　要：转Bt基因抗虫棉在沙培试验中对卡那霉素不同浓度的反应进行了检测。结果表明 ,浓度为 40 0 0mg L的卡那霉素液对非抗虫棉最为敏感 ,反应率为 1 0 0 % ,而转Bt基因抗虫棉均无反应。说明棉花室内抗卡那霉素反应能够准确检测转Bt基因抗虫棉纯度 ,可以作为一种快速、准确、简便的室内检测转Bt基因抗虫棉的方法。同时 ,不同浓度的卡那霉素液对棉苗胚轴。A simple,cheap,and efficient technique for transgenic Bt cotton using k anamycin as an indirect identification marker in the laborotary is tested.The re sult showed that kanamycin could be used in the laborotary to test whether or no t a plant is resistant.Seed of transgenic Bt cotton were cultured on sand bed co ntaining 4000 mg/L kanamycin solution for six days.The cotyledons of resistant v a rieties remained normal green color,whilst these of the commercial varieties all turned yellow.This technique is especially usful for the purity test of transge nic Bt cotton before the seed is sold. Meanwhile,the hypocotyls and fresh weigh of the cotton seedling were affected by the kanamycin in some degree.

关 键 词：转BT基因抗虫棉 室内鉴定技术 沙培试验 卡那霉素 浓度 反应率 

分 类 号：S562[农业科学—作物学]