泰和乌骨鸡的核型与带型研究  

Studies on Ikihe Silkies' Karyotype and Band Pattern

作　　者：陈国宏[1] 刘莉[1] 张学余[2] 李碧春[1] 吴信生[1] 徐琪[1] 

机构地区：[1]扬州大学畜牧兽医学院动科系,江苏扬州225009 [2]江苏省家禽科学研究所,江苏扬州225003

出　　处：《遗传》2003年第4期401-408,共8页Hereditas（Beijing)

基　　金：国家高新技术发展计划项目(863-2001AA243083);国家自然科学基金(30170673)

摘　　要：采用外周血淋巴细胞培养——空气干燥法,对泰和乌骨鸡染色体核型和带型进行了研究。结果表明:泰和乌骨鸡体细胞染色体数目2n=78,染色体基本臂数AF=90,1、9号染色体及Z、W性染色体为中央着丝粒(m)染色体,2、4、7号染色体为亚中央着丝粒(sm)染色体,3、6、8、10号染色体为端着丝粒(t)染色体。G带研究表明:前10对大型染色体可分为29个区,190条带。C带处理发现,所有母鸡分裂相中W性染色体都出现C带并整条深染。Ag-NORs研究发现:Ag-NORs常定位于1、2号常染色体短臂和Z性染色体短臂端部;Ag-NORs数目分布范围为1～6;平均每个细胞的Ag-NORs数在雌、雄鸡中分别为2.94和2.96。This study made the chromosome slides of Taihe Silkies by the peripheral blood lymphocyte culture-drying method,and analyzed Taihe Silkies karyotype and band pattern. The results are as follow:The diploid chromosome number of Taihe Silkies was 2n=78,the basic number of chromosome arms was AF=90 and the sex chromosome type was ZZ(♂ )/ZW(♀). According to the measured relative length, arm ratio and centromeric index, the first 10 pairs of macro-chromosomes are described as follows:No. 1 ,9 and Z,W chromosomes were metacentrics,No. 2,4,7 were submetacentrics,and No. 3,6,8,10 were telocentrics. Studies on Taihe Silkies G-band showed that the first 10 pairs of macro-chromosomes can be divided into 29 zones and 190 bands. Being treated by C-banding techniques totally dark-stained and easily indentified W-chromosome always showed up in the female metaphase configurations. Ag-NORs were located in the short arms' telomere of No. 1,2 euchromosomes and Z sexchromosome,the Ag-NORs number varied from 1-6.

关 键 词：泰和乌骨鸡 核型 带型 染色体 

分 类 号：S831[农业科学—畜牧学]