新疆三种雅罗鱼属鱼类mtDNA D-loop多态性及起源分化分析  

Polymorphism and Original Analysis of mtDNA D-loop of Three Leuciscus Species in Xinjiang

作　　者：胡文革[1] 王金富[2] 盛金良[1] 段子渊[3] 马润林[3] 

机构地区：[1]石河子大学生物工程学院,新疆石河子832003 [2]石河子大学分子生物学实验室,石河子832003 [3]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《遗传》2003年第4期414-418,共5页Hereditas（Beijing)

基　　金：新疆石河子大学分子生物学实验室经费资助

摘　　要：用PCR-RFLP技术,对新疆分布的准噶尔雅罗鱼、贝加尔雅罗鱼和高体雅罗鱼的mtDNA D-loop高变区约827bp进行了扩增,用ScaI、HinfI、AluI、DdeI4种限制性内切核酸酶对56个样本的扩增产物酶切和RFLP分析,共检测到6种单倍型。准噶尔雅罗鱼存在2种单倍型:BDAA和BDBA;贝加尔雅罗鱼存在3种:AAAA、ABAA和ACAA;高体雅罗鱼只有1种。初步认为,准噶尔雅罗鱼、贝加尔雅罗鱼存在较丰富的群体内变异。用6种单倍型及净遗传距离绘制的UPGMA分子系统树,提示准噶尔雅罗鱼有可能是原始种,贝加尔雅罗鱼和高体雅罗鱼是由准噶尔雅罗鱼进化而来。PCR-RFLP technique was employed to amplify about 827bp of mtDNA D-loop hypervariable region of Leu-ciscus baicalensis ,L. merzbacheri and L. idus in Xinjiang. The PCR products of 56 samples with four restriction enzymes were digested:Seal, HintI,AluI,Ddel,and RFLP analysis was done then. The study indicates that all of L. species and populations have six haplotypes, L. merzbacheri has two haplotypes: BDAA, BDBA; L. baicalensis has three: AAAA, ABAA, ACAA; L. idus has one:CAAA. It is primarily considered that L. merzbacheri and L. baicalensis have more intra-population mutations. The UPGMA trees with 6 haplotypes and net genetics distance pointed out that L. merzbacheri might be original species,L. baicalensis and L. idus are evolved from L. merzbacheri.

关 键 词：新疆 雅罗鱼 MTDNA D-100p PCR—RFLP 多态性 进化 

分 类 号：Q953[生物学—动物学]