电针上调食源性肥胖大鼠弓状核α-MSH的表达  

Electroacupuncture up-regulated arcuate nucleus α-MSH expression in the rat of diet-induced obesity

作　　者：田德润[1] 李晓东[1] 牛东滨[1] 石玉顺[1] 张肇康 韩济生[1] 

机构地区：[1]北京大学神经科学研究所 [2]美国加利福尼亚州凤凰公司

出　　处：《北京大学学报（医学版）》2003年第5期458-461,共4页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金 ( 3 0 2 70 62 4)资助~~

摘　　要：目的 :研究电针对食源性肥胖 (diet inducedobesity ,DIO)大鼠体重及摄食量的影响并探讨其中枢神经化学机制。方法 :通过高能饲料喂养 ,建立DIO大鼠模型 ,分为 :(1) 2Hz电针组 ,(2 ) 10 0Hz电针组 ,(3)束缚对照组 ,(4 )DIO组 ,(5 )肥胖抵抗 (dietresistance,DR)组 ,(6 )普通饲料对照组。电针参数 :0 .5、1 0、1.5mA各 10min。穴位 :双侧足三里和三阴交。每周电针 3次。每日记录体重及摄食量 ,共 4周。免疫组织化学方法观察下丘脑弓状核α 黑色素细胞刺激素 (α melanocyte stimulatinghormone ,α MSH)免疫阳性细胞数变化。结果 :与DR组和普通饲料对照组相比 ,DIO组的体重及摄食量增加 ,而α MSH免疫阳性细胞数则明显降低。与束缚对照组和DIO组相比 ,2Hz电针组、10 0Hz电针组的体重和摄食量明显降低 ,弓状核内α MSH免疫阳性细胞数明显增多。结论 :高能食物饲养导致的肥胖可能与下丘脑弓状核α MSH水平降低有关。电针治疗肥胖的机制之一 ,可能是通过刺激下丘脑弓状核前阿黑皮素 (pro opiomelanocortin ,POMC)神经元释放α MSH ,抑制摄食 。Objective: To investigate the effect of electroacupuncture (EA) on rat with diet induced obesity (DIO) and to explore the possible neurochemical mechanisms using the technique of immumohistochemisty. Methods:To establish DIO rat model by feeding the animals with high fat diet for 14 weeks. DIO rats were randomly divided into 4 groups: (1) 2Hz EA group, (2) 100Hz EA group, (3) restrain control group,(4) diet resistance (DR) group,(5) DIO group and (6) normal control group. EA treatment: (1) The acupoints used were Zusanli and Sanyinjiao on both legs. (2) The intensities of stimulation were 0.5, 1.0 and 1.5mA for 10 mins each. EA treatment was administered 3 times per week. Food intake and body weight were measured daily for 4 weeks. (3) The changes of the expression of α melanocyte stimulating hormone (α MSH) in the hypothalamic arcuate nucleus (ARC) were measured with immunohistochemical semiquantitative analysis. Results: (1) The food intake and body weight of 2 Hz EA group and 100 Hz EA group were decreased significantly compared with the restrain control group and DIO group. (2) The number of α MSH positive cells in hypothalamic ARC in 2 Hz EA and 100 Hz EA group was significantly higher than that in restrain control group and DIO group. The number of α MSH positive cells in hypothalamic ARC in DIO group is significantly lower than those in DR group or normal control group. Conclusion: A decrease of α MSH level in hypothalamus may be associated with diet induced obesity. The therapeutic effect on obesity produced by EA may be accounted for by the stimulation of pro opio melanocortin neurons in hypothalamic ARC to release α MSH, which inhibits food intake , resulting in a decrease of body weight.

关 键 词：肥胖症 电针 α-促黑素 弓状核 神经肽类 

分 类 号：R589.2[医药卫生—内分泌] R454.1[医药卫生—内科学]