HCV全长基因真核表达载体的构建及其细胞内表达  

Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in Mammalian Cells

作　　者：焦成松[1] 王升启[2] 张贺秋[2] 凌世淦[2] 

机构地区：[1]广州军区武汉总医院感染内科,湖北武汉430070 [2]军事医学科学院基础医学研究所,北京100850

出　　处：《中国病毒学》2003年第6期538-540,T006,共4页Virologica Sinica

基　　金：国家博士后基金;湖北省自然科学基金(2003ABA157)

摘　　要：本实验成功构建了HCV全长基因的真核表达载体pCI-HCVFL,转染HepG2细胞后经免疫荧光和免疫组化法分别检测到结构基因区(C)和非结构基因区(NS3)病毒蛋白的表达,该栽体可用于建立HCV转基因细胞模型以及进一步开展有关HCV复制与表达的深入研究。An eukaryotic vector containing the full-length Hepatitis C virus(HCV) gene, pCI-HCV, was successfully constructed in this study. After transfected into HepG2 cells, it was revealed to express HCV C and NS3 proteins in the cloned cells by immunofluorescence and immunohistochemistry assay respectively. It could be used for the experiments of the construction of HCV transgenic cellular model and the further study on the replication of HCV through cDNA.

关 键 词：丙型肝炎病毒 全长基因 蛋白表达 表达载体 

分 类 号：R346[医药卫生—基础医学]